低功率激光对细胞质膜通透性及细胞功能的影响

目的:探索低功率激光对细胞质膜通透性及细胞功能的影响。方法:以波长为632.8nm,功率密度为5.4mW/cm~2的氦氖激光照射人外周血淋巴细胞15、30、60分钟,并采用钙荧光指示剂Fura—2/Am定量测试法检测淋巴细胞内游离钙浓度和质膜Ca~(2+)—Mg~(2+)—ATP酶活性变化。结果:照射后淋巴细胞内游离钙浓度明显低于正常(P<0.05);同时细胞膜Ca~(2+)—Mg~(2+)—ATP酶活性增加(P<0.05);而且照射后细胞内游离钙浓度降低与质膜上Ca~(2+)—Mg~(2+)—ATP酶的激活呈负相关。结论:低功率激光照射激活细胞膜Ca~(2+)—Mg~(2+)—ATP酶活性,使细胞膜对钙通透性发生变化,且影响到细胞内Ca~(2+)贮存,造成细胞膜通透性和细胞功能的改变。

The Effect of Cytoplasmic Free Calcium Concentration and Plasma Membrance Ca2+- Mg2+ -ATPase Activity by Low Power Density Laser Irradiation on Human Peripheral Blood Lymphocytes

Objective: To investigate the effect of low power density laser irradiation on cytoplasmic free calcium concentration and the change of plasma membrane Ca2+ - Mg2+ - ATPase activity of human peripheral blood lymphocytes. Methods: Human peripheral blood lymphocytes, in vitro, randomized into irradiated group or control group. Lymphocytes were exposed to low power density He-Ne laser for 15, 30, 60min, and then the cytoplasmic free calcium concentration in human peripheral blood lymphocytes were measured by fluorescent indicator Fura-2/ Am estimating technique and the change of plasma membrane membrane Ca2+ - Mg2+ - ATPase activity in lymphocytes were measured by Lowry's technique. Results: The concentration of intracellular plasmic free calcium was lower in irradiated group than in control group (P<0. 05) , and the activity of plasma membrane Ca2+ -Mg2+ -ATPase increased significantly. Those changes reduced along with the length of exposing time. It showed that the concentration of intracellular free calcium correlated with the change of plasma membrane Ca2+ - Mg2+ - ATPase activity. Conclusion: The acticity of plasma membrane Ca2+ - Mg2+ -ATPase was activated by low power density laser irradiation of certain energy, and then followed by the change of permeability of plasma membrane, influencing the change of storage of intracellular free calcium and the change of permeability of cell membrane and also cellular function .