Selective adsorption and separation of adenine by molecularly imprinted polymethacrylic acid on surface of silica particles |
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| Authors: | Yang-Chun Liu Chao Tian Hai-Lin Cong Qiao-Hong Peng Shen-Hong Xu |
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| Affiliation: | 1. Institute of Biomedical Materials and Engineering, College of Chemistry and Chemical Engineering, Qingdao University, Qingdao, China;2. Laboratory for New Fiber Materials and Modern Textile, Growing Base for State Key Laboratory, College of Materials Science and Engineering, Qingdao University, Qingdao, China |
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| Abstract: | ABSTRACTAdenine molecular imprinted polymethacrylic acid (PMAA) was in situ prepared on the surface of silica (SiO2) microspheres, constituting a thin and rough molecular imprinted polymer (MIP) layer as characterized by SEM, TEM and FT-IR. Dynamic method and competitive adsorption experiments were used to investigate the adsorption kinetics, adsorption isotherms, selective recognition and adsorption reproducibility of MIP-PMAA/SiO2 particles toward adenine. The results showed that the obtained particles have strong selective affinity, specific recognition and excellent reusability for adenine. MIP-PMAA/SiO2 particles were further used as stationary phase for high performance liquid chromatography (HPLC) and separation of adenine from other nucleic bases was achieved successfully. |
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| Keywords: | Adenine molecularly imprint polymethacrylic acid silica |
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