以玉米芯为原料酶法制备低聚木糖的研究

以玉米芯为原料,在固液比1：10,NaOH 质量浓度4%,50℃条件下处理24h,木聚糖提取率为91．0%。利用木聚糖酶降解木聚糖制备低聚木糖,确立了最佳酶解工艺条件为：50℃,pH 4．8,木聚糖底物质量浓度 3．0%,每克底物的木聚糖酶用量为50IU,反应时间0．5h。在上述反应条件下,产品平均聚合度为3．61,低聚木糖得率为91．2%。该研究结果在可再生半纤维素资源利用方面具有重要的意义。

Production of Xylooligosaccharides from Corncobs by Xylanase Hydrolysis

Xylooligosaccharides production was carried out by enzymatic hydrolysis of xylan,which was ob- tained from corncobs treated with 4% NaOH solution at liquid-to-solid ratio 10:1 for 24 hours at 50℃.The yield of xylan extracted from corncob was 91.0%.The optimized process conditions for xylooligosaccharides production were as follows:reaction temperature 50℃,pH value 4.8,xylan substrate concentration 3.0%,xylanase amount,50IU/g substrate,hydrolysis time 0.5 hours.The average degree of polymerization (DP) of the product was 3.61 and the total yield of xylooligosaccharides reached 91.2%.The results showed that the potential applica- tion of renewable hemicellulosic resource.