超氧化物歧化酶与阿米福汀对小鼠急性放射损伤预防作用的比较

本研究建立了小鼠全身放疗的损伤模型,以比较预给药超氧化物歧化酶(SOD)和阿米福汀对小鼠放射治疗损伤的防护效果。C57BL/6小鼠用6 Gy X射线进行全身一次性照射,在放射后24 h处死,测定小鼠外周血红、白细胞数量,胸腺指数和脾脏指数,以及肝脏抗氧化活力。结果表明:与放疗对照组相比,放疗前给药SOD组对放疗鼠的外周血白细胞数量和胸腺指数有显著的保护作用,使之分别增加66.7%和19.1%。测得放疗前给药阿米福汀对放射鼠的外周血白细胞数量和脾脏指数有显著的保护作用,使之分别增加106.9%和22.6%。此外,与放疗对照组相比,放疗前给药SOD对放疗鼠的肝脏抗氧化能力也有显著提高,可使肝脏的MDA水平下降12.7%,SOD活力增加10.8%,T-AOC活力增加45.0%,放疗前给药阿米福汀可使放疗鼠肝脏的MDA水平下降22.3%。

Investigating preventive effects of superoxide dismutase and amifostine on acute radiation injury of mice

The radiation injury models of mice were established and the preventive effects of superoxide dismu-tase（SOD） compared with amifostine from radiation damage were investigated.C57BL/6 mice were whole-body ir-radiated with 6 Gy X-rays and sacrificed by cervical dislocation at 24 h after irradiation.The red blood cells and white blood cells counts in peripheral blood,thymus index,spleen index,and hepatic antioxidant activity were determined,respectively.The results show that compared with the group received irradiation only,SOD pretreatment has signifi-cant effect on white blood cells counts meanwhile thymus index is significantly elevated by 19.1%.Amifostine pre-treatment has also significant effect on white blood cells counts and spleen index is significantly elevated by 22.6%.In addition,SOD pretreatment can significant protect hepatic antioxidant activity and decrease nalondialdehyde（MDA） level by 12.7% in irradiated while the total anti-oxidizing capability（T-AOC） is up by 45.0%.However,amifostine treatment could only remarkably decreased MDA level by 22.3%.