早熟葡萄胚抢救技术研究

针对早熟葡萄育种中胚发育不全的问题,以京秀×87-1等5个杂交组合的杂交胚为材料,采用L25 (55)的正交试验设计,进行了早熟葡萄胚抢救技术研究。结果表明,影响各个组合早熟葡萄杂交后代成苗率最重要的因素是取胚时期,其他的因素对成苗率的影响大小因基因型而异。筛选出了早熟葡萄胚挽救的最优培养时期、相对较优的基本培养基成分及几种激素浓度,积累了一批胚抢救杂交试材。

Research on embryo rescue technology in early-ripening grape

To solve the question of incomplete development of embryo in early-ripening grape breeding, orthogonal experimental design of L25( 55 )was adopted to study the embryo rescue technology in early- ripening grape with the crossing embryos of 5 crossing combinations as materials. The results indicated that the sampling date was the most important factor that affected the ratio of seedlings of early- ripening grapes. Effects of other factors to the ratio of seedlings were different because of different genotypes. This experiment screened out the optimal culture date, relative optimal basal medium components with concentrations of several kinds of hormones, and obtained a set of hybridization materials from embryo rescue.