PEI对固定化增殖细胞性质的影响

以海藻酸钙为载体包埋黑曲霉AS0 0 2 3孢子 ,培养后得到的固定化增殖细胞用于生产低聚果糖 .利用材料试验仪研究了聚乙烯亚胺 (PEI)对固定化增殖细胞强度的影响 ,并研究了PEI对固定化增殖细胞酶活及最适 pH值的影响 .结果表明 ,经PEI处理后 ,尽管固定化增殖细胞酶活略有降低 ,最适 pH值由 5.5降至 4 .0 ,但凝胶强度有明显改善 .起始机械强度由 0 .0 6N/粒增加到 0 .2 79N/粒 ,而机械强度衰减率仅为未处理组的 9.2 7% .

Effect of Polyethyleinimine on Properties of Immobilized Growth Cells

The spores of Aspergillus niger AS0023 were entrapped in calcium alginate gels for growth to form immobilized growth cells which were used for the production of fructooligosaccharides(FOS).The effect of polyethyleinimine(PEI) on the gel strength of immobilized growth cells was measured by Lloyd 1 instrument 1000S. The effects of PEI on its enzyme activity and on the optimum pH were also studied. The results showed that with the treatment of PEI, the gel strength improved obviously , although the enzyme activity of immobilized growth cells decreased a little and the optimum pH changed from 5.5 to 4.0. The initial mechanical strength increased from 0.06 N/bead to 0.279 N/bead, and the decreasing ratio of mechanical strength was 9.27% comparing with the group without treatment of PEI.