| 海洋假单胞菌胞外多糖的抗氧化性 |
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| 引用本文： | 孙红梅,张星星,白玉,王际辉,王晗,肖珊,叶淑红.海洋假单胞菌胞外多糖的抗氧化性[J].大连工业大学学报,2012(6):395-398. |
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| 作者姓名： | 孙红梅 张星星 白玉 王际辉 王晗 肖珊 叶淑红 |
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| 作者单位： | 大连工业大学辽宁省食品生物技术重点实验室 |
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| 基金项目： | 国家农业科技成果转化资金项目(2010GB2B000092);国家星火计划项目(2010GA651004);辽宁省高等学校优秀人才支持计划项目(LR2011012);辽宁省高等学校优秀人才支持计划项目(LJQ2011055);辽宁省科学技术基金项目(201202013);大连市科技计划项目(2012B14NC128) |
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| 摘 要： | 利用海洋假单胞菌PT-8发酵胞外多糖,对其发酵液进行乙醇沉淀,Sevag法除蛋白,透析、冷冻干燥后得到纯化的胞外多糖(EPSs)。用分光光度法测定多糖的还原力以及在不同体系中对羟自由基、DPPH自由基和超氧阴离子自由基的清除能力。结果表明,多糖具有较强的还原力、羟自由基和超氧阴离子的清除能力,多糖对DPPH的清除作用较弱,低于同等条件下的维生素C。
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| 关 键 词： | 假单胞菌 胞外多糖 抗氧化性 |
Antioxidant effects of extracellular polysaccharides from a marine Pseudomonas |
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| SUN Hong-mei,ZHANG Xing-xing,BAI Yu,WANG Ji-hui,WANG Han,XIAO Shan,YE Shu-hong.Antioxidant effects of extracellular polysaccharides from a marine Pseudomonas[J].Journal of Dalian Dalian Polytechnic University,2012(6):395-398. |
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| Authors: | SUN Hong-mei ZHANG Xing-xing BAI Yu WANG Ji-hui WANG Han XIAO Shan YE Shu-hong |
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| Affiliation: | (Liaoning Provincial Key Laboratory of Food Biotechnology,Dalian Polytechnic University,Dalian 116034,China) |
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| Abstract: | A marine pseudomonas PT-8 was fermented to produce extracellular polysaccharides(EPSs).The fermentation liquor was treated in alcohol precipitation; Sevag method was used to remove protein, dialysis, freezing and drying method was used to purified EPSs.The scavenging effects on·OH, DPPH radical and O 2·by EPS were investigated using spectrophotometry.The results indicated that the EPSs had strong reducing power and good scavenging ability to the ·OH and O 2·.Its ability of scavenging DPPH radical was much lower than that of VC under the same conditions. |
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| Keywords: | pseudomonas extracellular polysaccharide a ntioxidation |
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