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透射电镜电子染色方法的改良
引用本文:孟丽,崔芳,龚淼,武惠珍. 透射电镜电子染色方法的改良[J]. 电子显微学报, 2010, 29(2): 189-190
作者姓名:孟丽  崔芳  龚淼  武惠珍
作者单位:河北医科大学,电镜实验中心,河北,石家庄,050017;河北医科大学,组胚教研室,河北,石家庄,050017;河北医科大学,实验诊断教研室,河北,石家庄,050017
摘    要:为提高透射电镜生物样品超薄切片的染色效率,在传统染色方法基础上进行改良,使用自制染色装置,在较短时间内完成大批生物样品超薄切片的电子染色。与传统方法相比较省时省药,减少污染机率,电镜观察的超微结构清晰,反差较好。

关 键 词:透射电镜  电子染色  方法改良

Improvement of staining method for transmission electron microscopy
MENG Li,CUI Fang,GONG Miao,WU Hui-zhen. Improvement of staining method for transmission electron microscopy[J]. Journal of Chinese Electron Microscopy Society, 2010, 29(2): 189-190
Authors:MENG Li  CUI Fang  GONG Miao  WU Hui-zhen
Affiliation:1.Laboratory Center of Electron Microscopy;2.Department of Histology and Embryology;3.Department of Laboratory Diagnosis;Hebei Medical University;Shijiazhuang Hebei 050017;China
Abstract:For the improvement of the biological specimens staining efficiency of transmission electron microscopy,we used self- commanding staining devices which based on traditional staining method,and complete a large number of electron staining of biological specimens within short time.Compared with traditional method,the new method could save time and drug,and reduce the probability of stain contamination.The ultrastructure results observed by electron microscope were more distinct and contrasty.
Keywords:transmission electron microscope  electron staining  improvement of method  
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