| 壳聚糖固定化纤维素酶的条件优化 |
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| 引用本文： | 张孟麒,林增祥,张红漫,黄和.壳聚糖固定化纤维素酶的条件优化[J].中国酿造,2010(1). |
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| 作者姓名： | 张孟麒 林增祥 张红漫 黄和 |
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| 作者单位： | 1. 南京工业大学,生物与制药工程学院,江苏,南京,210009
2. 南京工业大学理学院,江苏,南京,210009 |
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| 基金项目： | 国家自然科学基金，国家高技术研究发展计划'863计划'，NSFC-广东联合基金项目 |
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| 摘 要： | 采用Plackett-Burman试验设计和正交试验设计对影响固定化纤维素酶活的因素进行了筛选和优化.探讨了纤维素酶、壳聚糖、乙酸、乙醇、戊二醛、氢氧化钠等试剂对固定化效果的影响,筛选出制备固定化纤维素酶过程中的主要影响因素.结果表明:壳聚糖、乙酸、乙醇浓度、酶浓度分别为15g/L、2.0%、40%、100%时,以壳聚糖为载体制备固定化纤维素酶的活力最高,成型效果最好.
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| 关 键 词： | 纤维素酶 崮定化 Plackett-Burman设计 正交试验 |
Optimization of processing parameters of cellulase immobilization on chitosan beads |
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| ZHANG Mengqi,LIN Zengxiang,ZHANG Hongman,HUANG He.Optimization of processing parameters of cellulase immobilization on chitosan beads[J].China Brewing,2010(1). |
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| Authors: | ZHANG Mengqi LIN Zengxiang ZHANG Hongman HUANG He |
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| Affiliation: | ZHANG Mengqi1,LIN Zengxiang1,ZHANG Hongman2,HUANG He1 (1.College of Biotechnology , Pharmaceutical Engineering,Nanjing University of Technology,Nanjing 210009,China,2.College of Science,China) |
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| Abstract: | Screening and optimization of key factors for cellulase immobilization on chitosan bead was studied by Plackett-Burman design and the orthogonal experiment.The main factor was determined by study on effects of chitosan,acetic acid,ethanol,sodium hydroxide,glutaraldehyde and cellulase on the activity of cellulose immobilized on chitosan.The result showed that the highest enzyme activity and best mechanic strength of immobilized cellulose occurred when the concentration of chitosan,acetic acid and ethanol are... |
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| Keywords: | cellulase immobilization Plackett-Burman design orthogonal experiment |
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