利用活性干酵母发酵生产富硒酵母

对活性干酵母(ADY)的耐Se、富Se性能作了测试，并初步研究了利用ADY摇瓶发酵生产富Se酵母的培养条件.结果表明ADY具有很强的耐Se、富Se能力；无机Se添加量和培养时间等，是影响其Se转化能力的主要因素.在适宜的摇瓶发酵条件(麦芽汁12°Be,加Se量15mg/L,装量60mL/250mL三角瓶,pH=6.0，30℃，30h)下，富Se酵母干重、有机Se含量和Se转化率分别可达15.8g/L，790.0μg/g和83.2%.

Production of selenium yeast by active dry yeast

The selenium-rich and selenium-tolerant ability of active dry yeast(ADY) and i ts conditions of bioconversion of inorganic selenium to the organic seleniun wer e studied. The results showed that ADY has strong selenium-rich and selenium- tolerant ability and the culture conditions have a great influence on selenium-rich ability.The concentraction of inorganic-sele nium in medium and culture time,etc., were main factors affecting bioconversion of inorganic selenium. Under the initial optimum conditions(the mash liquor of 1 2 °Be, the inorganic-selenium concentraction of 15 mg/L,medium of 60 mL in a 2 50 mL flask, initial pH=6.0, and culture time 30 h at 30 ℃), the dry weight of selenium yeast and the content of organic selenium in the cells as well as the r ate of se-bioconversion were 15.8　g/L,790.0 μg/g and 83.2% in proper order.