啤酒花苦味酸对血糖调节作用的研究

观察啤酒花超临界CO2 浸膏、β- 酸和六氢β- 酸对糖尿病小鼠血糖的调节作用。对四氧嘧啶复制的糖尿病小鼠的动物模型进行为期1w 的啤酒花超临界CO2 浸膏、β- 酸和六氢β- 酸的高、低剂量(30、15mg/kg)组和阳性药物格列本脲片灌胃治疗，观察小鼠的糖尿病“三多一少”的症状和治疗前后血糖和糖耐量的变化。与模型对照组相比，治疗组糖尿病小鼠的“三多一少”症状得到明显地改善，治疗前后血糖和糖耐量的变化结果表明，超临界CO2 浸膏和β- 酸的高剂量组治疗后的血糖值与正常组(p ＞ 0.05)无明显差异。对治疗后小鼠的血糖值采用单一自由度正交比较的方差分析表明，正常组小鼠的血糖值与模型组有极显著的差异，模型对照组与治疗组之间也具有非常显著的差异(p ＜ 0.01)；在各治疗组之间，灌胃优降糖片的小鼠的治疗效果与灌胃不同剂量的β- 酸、六氢β- 酸和超临界CO2 浸膏样品的小鼠的总体治疗效果相当(p ＞ 0.05)。啤酒花超临界CO2 浸膏、β- 酸和六氢β- 酸对小鼠糖尿病症状均有一定的治疗作用，该作用与灌胃样品的剂量呈正相关关系。

Study on Modulation Effects of Hop Acids on Blood Glucose Level of Alloxan-induced Diabetic Mice

To investigate the modulation effects of supercritical CO2 extracts of hop, β-acids and hexahydro-β-acids on blood glucose level in diabetic mice, an animal model with alloxan-induced diabetic mellitus was established, and the symptom change of diabetes mellitus was observed before and after a 7-day gastric perfusion therapy with supercritical CO2 extracts of hop, β- acids, hexahydro-β-acids and glybenzoylamide (a control group). The results suggested that the fasting blood glucose level and oral glucose tolerance are reduced after therapy, especially the blood glucose level in mice treated with the high dose of β-acids and supercritical CO2 extracts of hop is similar to that of normal mice (p ＞ 0.05). Moreover, the single factor orthogonal comparison showed that the blood glucose level is significantly different between normal and model groups or between model contrast and model therapy groups (p ＜ 0.01). Among therapy groups, supercritical CO2 extracts of hop, β-acids and hexahydro-β-acids have similar effect to glibenclamide (p＞0.05), and their therapy effects on mouse blood glucose level are positively related to their doses.