Characteristics of DNA Comet, Photostimulated Luminescence, Thermoluminescence and Hydrocarbon in Perilla Seeds Exposed to Electron Beam |
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| Authors: | H-W Chung H Delincée S-B Han J-H Hong H-Y Kim J-H Kwon |
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| Affiliation: | 1Authors Chung and Kwon are with the Dept. of Food Science and Technology, Kyungpook National Univ., Daegu 702-701, Korea. Author Delincée is with the Institute of Nutritional Physiology, Federal Research Centre for Nutrition, Haid-und-Neu-Str. 9, D-76131 Karlsruhe, Germany. Authors Han, Hong and Kim are with the Div. of Food Standard, Dept. of Food Evaluation, The Korea Food &Drug Administration, 5, Nokbun-dong, Eunpyunggu, Seoul 122-704, Korea. Direct inquiries to author Kwon (E-mail: ). |
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| Abstract: | ABSTRACT: Detection characteristics of perilla seeds exposed to electron beam doses of 0 to 2 kGy were investigated. DNA comet assay, photostimulated luminescence (PSL) and thermoluminescence (TL) measurements, and hydrocarbon determinations by GC-MS were carried out. The irradiated samples showed comets with longer tails (152 ± 6 mm) than did nonirradiated samples (35 ± 11 mm). PSL values (photon counts) for the irradiated samples were more than 7865; whereas those of nonirradiated samples were less than 294. TL ratios (Glow 1/Glow 2) obtained from nonirradiated samples were less than 0.01 and above 0.48 from irradiated ones. The prominent radiation-induced hydrocarbons were 16:2, 16:3, 17:1, and 17:2, of which 16:2 was the most abundant. |
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| Keywords: | perilla seeds irradiation detection DNA comet assay PSL TL GC-MS |
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