Osteogenic activity of human periosteal sheets cultured on salmon collagen-coated ePTFE meshes |
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Authors: | Tomoyuki Kawase Kazuhiro Okuda Hiroyuki Kogami Hitoshi Nakayama Masaki Nagata Hiromasa Yoshie |
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Affiliation: | (1) Division of Oral Bioengineering, Department of Tissue Regeneration and Reconstitution, Institute of Medicine and Dentistry, Niigata University, 951-8514 Niigata, Japan;(2) Division of Periodontology, Department of Oral Biological Science, Institute of Medicine and Dentistry, Niigata University, 951-8514 Niigata, Japan;(3) Japan Science and Technology Agency Innovation Satellite Niigata, 940-2127 Nagaoka, Japan;(4) Division of Oral and Maxillofacial Surgery, Department of Oral Health Science, Institute of Medicine and Dentistry, Niigata University, 951-8514 Niigata, Japan |
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Abstract: | Our animal implantation studies have demonstrated that, after osteogenic processing, cultured human periosteal sheets form
osteoid tissue ectopically without the aid of conventional scaffolding materials. To improve the osteogenic activity of these
periosteal sheets, we have tested the effects of including a scaffold made of salmon collagen-coated ePTFE mesh. Periosteal
sheets were produced with minimal manipulation without enzymatic digestion. Outgrown cells penetrated into the coated mesh
fiber networks to form complex multicellular layers and increased expression of alkaline phosphatase activity in response
to the osteoinduction. In vitro mineralization was notably enhanced in the original tissue segment regions, but numerous micro-mineral
deposits were also formed on the coated-fiber networks. When implanted subcutaneously into nude mice, periosteal sheets efficiently
form osteoid around the mineral deposits. These findings suggest that the intricate three-dimensional mesh composed of collagen-coated
fibers substantially augmented the osteogenic activity of human periosteal sheets both in vitro and in vivo. |
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