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Continuous glucose monitoring using enzyme-immobilized platinized diamond microfiber electrodes |
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| Authors: | H Olivia K Honda |
| Affiliation: | a Department of Applied Chemistry, The University of Tokyo, 113-8656 Tokyo, Japan b Department of Chemistry and Earth Science, Yamaguchi University, 753-8512 Yamaguchi, Japan c Kanagawa Advanced Science and Technology (KAST), KSP Bldg. West 614, 3-2-1 Sakado, Takatsu-ku, Kawasaki-shi, 213-0023 Kanagawa, Japan |
| Abstract: | A sensitive and stable glucose biosensor for in vivo monitoring has been developed using boron-doped diamond microfiber (BDDMF) electrodes. The electrodes were modified with platinum nano-particles to detect H2O2, which was enzymatically produced by glucose oxidase (GOx) immobilized on the electrode surface. The platinum-modified BDDMF (Pt-BDDMF) electrodes exhibited much higher sensitivity compared to Pt-microfiber electrodes, Pt electrodes and Pt-modified diamond thin film electrodes. Deposition conditions for Pt nano-particles on the BDDMF electrodes and immobilization of GOx were optimized. GOx/overoxidized polypyrrole (OPPy)/Pt-modified BDDMF electrodes were applied for continuous interference-free glucose monitoring. Amperometric measurements of glucose showed a linear response in the range of 1-70 mM, with an R.S.D. of 3.7% for five injections of 100 μM glucose. The electrodes exhibited good stability over 3 months with no detected anodic current for ascorbic acid (AA), which is an interfering compound. |
| Keywords: | Diamond Microfiber electrode Platinized Glucose Enzymatic H2O2 |
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