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| 黄浆水中高产γ-氨基丁酸乳酸菌的筛选及鉴定 | |
| 引用本文： | 刘佳荣,梁金钟. 黄浆水中高产γ-氨基丁酸乳酸菌的筛选及鉴定[J]. 食品科学, 2014, 35(23): 221-226. DOI: 10.7506/spkx1002-6630-201423043 |
| 作者姓名： | 刘佳荣 梁金钟 |
| 作者单位： | 哈尔滨商业大学食品工程学院，黑龙江省高校食品科学与工程重点实验室，黑龙江 哈尔滨 150076 |
| 基金项目： | 黑龙江省高校科技创新团队建设计划项目(2010td04) |
| 摘 要： | 经初筛、复筛，从黄浆水中筛得一株高产γ-氨基丁酸（γ-aminobutyric acid，GABA）的菌株，对其进行形态学及生理生化鉴定，并与GenBank上已提交的16S rDNA进行BLAST比对，结果表明，其归属于乳酸杆菌属（Lactobacillus）。由MEGA 6.0软件构建的系统发育树结果表明，该菌株与Lactobacillus plantarum 16S rDNA序列同源性达99%，且与生理生化实验结果一致，因此，确定该菌株为植物乳杆菌（Lactobacillus plantarum），编号为LP-Dfa301，测得其发酵液中GABA产量为5.833 g/L。 |
| 关 键 词： | &gamma -氨基丁酸 乳酸菌 黄浆水 16S rDNA 系统发育树 |
Screening of Lactic Acid Bacteria with High Yield of Gamma-Aminobutyric Acid from Yellow Serofluid,the Wastewater from Tofu Production |
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| LIU Jia-rong,LIANG Jin-zhong. Screening of Lactic Acid Bacteria with High Yield of Gamma-Aminobutyric Acid from Yellow Serofluid,the Wastewater from Tofu Production[J]. Food Science, 2014, 35(23): 221-226. DOI: 10.7506/spkx1002-6630-201423043 | |
| Authors: | LIU Jia-rong LIANG Jin-zhong |
| Affiliation: | Key Laboratory of Food Science and Engineering, School of Food Engineering, Harbin University of Commerce,Harbin 150076, China |
| Abstract: | After primary screening and rescreening, a high-yield strain producing γ-aminobutyric acid (GABA) was isolated from yellow serofluid, the wastewater from tofu production. Morphology analysis, physiological and biochemical properties and 16S rDNA sequences in GenBank indicated that the strain belonged to the Lactobacillus genus. Then, the phylogenic tree generated by MEGA 6.0 software revealed that it was closely related to Lactobacillus plantarum with a similarity of 99%, and numbered LP-Dfa301. In the fermentation broth, GABA production could reach 5.833 g/L. |
| Keywords: | γ-aminobutyric acid (GABA) Lactobacillus yellow serofluid 16S rDNA phylogenic tree |
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