| 大粒车前子麸壳多糖的分离纯化及部分理化性质 |
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| 引用本文： | 施晓丹,林慧霞,殷军艺,聂少平.大粒车前子麸壳多糖的分离纯化及部分理化性质[J].食品科学,2014,35(23):39-42. |
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| 作者姓名： | 施晓丹 林慧霞 殷军艺 聂少平 |
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| 作者单位： | 1.南昌大学 食品科学与技术国家重点实验室，江西 南昌 330047；2.厦门华厦职业学院，福建 厦门 361000 |
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| 基金项目： | 国家高技术研究发展计划(863计划)项目(2013AA102102);江西省高等学校科技落地计划项目(KJLD13004);国家自然科学基金青年科学基金项目(31301434);2013年度高等学校博士学科点专项科研基金项目(20133601120009) |
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| 摘 要： | 以大粒车前子麸壳为研究对象，提取水溶性多糖组分并对其部分理化性质进行分析。车前子麸壳水提粗多糖经过SephacrylTMS-400 HR葡聚糖凝胶柱得到纯化的多糖组分（polysaccharide from bran of Plantago asiatica L.，PBPL）。结果显示：PBPL为均一多糖，平均分子质量为619 651 D，糖含量、蛋白质含量和糖醛酸含量依次为（71.420±0.007）%、（6.07±0.02）%、（20.74±0.05）%。PBPL含有鼠李糖、阿拉伯糖、甘露糖、葡萄糖及半乳糖5 种单糖，物质的量比为2.24∶1.87∶1.00∶3.24∶3.49，糖醛酸主要是半乳糖醛酸。红外光谱中有多糖、蛋白、糖醛酸以及β-吡喃糖环特征吸收峰。
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| 关 键 词： | 大粒车前子麸壳 多糖 理化性质 |
Isolation,Purification and Partial Physico-chemical Properties of Polysaccharide from Bran of Plantago asiatica L. |
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| SHI Xiao-dan,LIN Hui-xia,YIN Jun-yi,NIE Shao-ping.Isolation,Purification and Partial Physico-chemical Properties of Polysaccharide from Bran of Plantago asiatica L.[J].Food Science,2014,35(23):39-42. |
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| Authors: | SHI Xiao-dan LIN Hui-xia YIN Jun-yi NIE Shao-ping |
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| Affiliation: | 1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China;
2. Xiamen Huaxia Vocational College, Xiamen 361000, China |
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| Abstract: | A polysaccharide fraction, PBPL, purified from Plantago asiatica L. bran was characterized for physico-chemical
properties. It was extracted with hot water and purified by SephacrylTMS-400 HR chromatographic column. The relative
average molecular weight determined by high performance gel permeation chromatography (HPGPC) was 619 651 D. The
results showed that PBPL contained (71.420 ± 0.007)% of total sugar, (6.07 ± 0.02)% of protein and (20.74 ± 0.05)%
of uronic acid by a colorimetric method. Rhamnose, arabinose, mannose, glucose and galactose were found to be the
compositional monosaccharides at a molar ratio of 2.24:1.87:1.00:3.24:3.49 in PBPL using gas chromatography (GC). After
reduction, galacturonic acid was confirmed to be the major component of uronic acid. As shown by infrared spectral (IR)
analysis, PBPL had characteristic absorption peaks of polysaccharide, protein and uronic acid and β-pyranose ring. |
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| Keywords: | Plantago asiatica L bran polysaccharide physico-chemical properties |
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