| 螺旋藻多糖提取新工艺的研究 |
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| 引用本文： | 章银良,李红旗,高峻,沈忠耀,林文翰,付宏征. 螺旋藻多糖提取新工艺的研究[J]. 食品与发酵工业, 1999, 25(2): 15-18 |
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| 作者姓名： | 章银良 李红旗 高峻 沈忠耀 林文翰 付宏征 |
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| 作者单位： | 1. 清华大学化工系,北京,100084
2. 北京医科大学天然与仿生药物国家重点实验室,北京,100083 |
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| 摘 要： | 提取螺旋藻多糖的传统工艺存在着流程长，操作复杂，有机溶剂消耗大等问题。采用三氯乙酸（ＴＣＡ）法代替Ｓｅｖａｇ法去除蛋白质，使改进后的提取工艺中蛋白质去除率增加，多糖损失率降低，流程缩短。通过用ＤＥＡＥ－Ｓｐｅｈａｒｏｓｅ和Ｓｅｐｈａｄｅｘ－Ｇ５０柱的分离纯化得到２个多糖组分。经测定，其相对分子质量分别为Ｍｒ１＝１２６００，Ｍｒ２＝１６６００。组分１的单糖组成：Ｄ－葡萄糖、Ｄ－甘露糖、Ｄ－半乳糖和葡萄糖醛酸；组分２的单糖组成：Ｄ－葡萄糖、Ｄ－木糖、Ｌ－鼠李糖和葡萄糖醛酸。
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| 关 键 词： | 螺旋藻 多糖 纯化 |
New Process for Separation & Purification of Polysaccharide from Spirulina platensis |
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| Zhang Yinliang,Li Hongqi,Gao Jun,Shen Zhongyao,Lin Wenhan,Fu Hongzheng. New Process for Separation & Purification of Polysaccharide from Spirulina platensis[J]. Food and Fermentation Industries, 1999, 25(2): 15-18 |
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| Authors: | Zhang Yinliang Li Hongqi Gao Jun Shen Zhongyao Lin Wenhan Fu Hongzheng |
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| Abstract: | New process was proposed to improve the traditional process. Protein removal ratio , polysaccharide yield, operation time, the amount of organic solution, etc, were compared in this paper. The TCA method, used to remove protein instead of Sevag method , not only reduces the loss of polysaccharide but also increase the removal ratio of the protein. The operation time of the new process was 5 h short comparing with the traditional one. Purified by DEAESepharose and SephadexG50 column, two polysaccharide fractions were obtained. The molecular weight of these two fractions were 12 600 and 16 000, and the composition of fraction 1 is Dglucose, Dmannose, Dgalactose, glucuronic acid, fraction 2 is Dglucose, Dxylose, Lrhommose and glucuronic acid respectively. |
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| Keywords: | Spirulina platensis polysaccharide separation and purification |
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