Prophylactic effect of Lactobacillus oral vaccine expressing a Japanese cedar pollen allergen |
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Authors: | Ohkouchi Kayo Kawamoto Seiji Tatsugawa Kenji Yoshikawa Noboru Takaoka Yuki Miyauchi Sayumi Aki Tsunehiro Yamashita Mitsuo Murooka Yoshikatsu Ono Kazuhisa |
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Affiliation: | Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima 739-8530, Japan. |
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Abstract: | Lactic acid bacteria (LAB) represent an attractive delivery vehicle for oral allergy vaccine because of their safety as a food microorganism as well as their potent adjuvant activity triggering anti-allergic immune response. Here, we report the generation of recombinant LAB expressing a major Japanese cedar pollen allergen Cry j 1 (Cry j 1-LAB), and their prophylactic effect in vivo. To facilitate heterologous expression, the codon usage in the Cry j 1 gene was optimized for the host LAB strain Lactobacillus plantarum by the recursive PCR-based exhaustive site-directed mutagenesis. Use of the codon-optimized Cry j 1 cDNA and a lactate dehydrogenase gene fusion system led to a successful production of recombinant Cry j 1 in L. plantarum NCL21. We also found that oral vaccination with the Cry j 1-LAB suppressed allergen-specific IgE response and nasal symptoms in a murine model of cedar pollinosis. |
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Keywords: | Allergen Codon optimization IgE Japanese cedar pollen Lactobacillus |
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