| Mutagenic Effects of BM302：GO112 Induced by Low-Energy Ion Beam Implantation |
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| 引用本文： | 赵世光,汤明礼,王军,王陶,王顺昌,吴跃进,余增亮.Mutagenic Effects of BM302：GO112 Induced by Low-Energy Ion Beam Implantation[J].等离子体科学和技术,2007,9(4):508-512. |
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| 作者姓名： | 赵世光 汤明礼 王军 王陶 王顺昌 吴跃进 余增亮 |
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| 作者单位： | Key Laboratory of Ion Beam Bioengineering,Institute of Plasma Physics, Chinese Academy of Sciences, Hefei 230031, China |
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| 基金项目： | Acknowledgments The authors would like to thank Jiangsu Jiangshang Pharmaceutical Co. Ltd. for providing B. megaterium and G. oxydans. |
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| 摘 要： | Mutant strains of GO112 and BM302 with a high 2-keto-L-gulonic acid （2KLG） transformation rate induced by ion beam implantation were separately and combinatorially compared with the original strains GO29 and BM80 to study the mutagenic effects of ion beam implantation. Both the sole GOl12 and mixed BM302：GOl12 demonstrated improved SNDH activity and 2KLG yield compared to the original strains. The mutant combinations of BM302：GOl12 showed a longer stationary phase and higher biomass than BM80：GO29. The mutant BM302 exhibited a stronger capacity to maintain a stable pH environment at mixed fermentation with Gluconobacter oxydans （G. oxydans） for 2KLG transformation and facilitated the growth of G. oxydans compared with the original strain BM80. The promotive capacity to L-sorbosone dehydrogenase （L-SNDH） from the supernate of BM302 was 1.6-fold higher than that of BM80. Genes encoded SNDH in GO29 and GOl12 were amplified and sequenced, and mutations including three transitions （CG →TA, CG →TA, GC → AT） and one transversion （AT→ TA） were confirmed from GO29 to GOl12. The corresponding amino acid was changed as Leu →Phe, Arg → Gln and Asn → Lys.
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| 关 键 词： | 离子梁植入诱导有机突变影响 生理学变异 突变 米格列醇 葡萄糖抑制剂 |
| 修稿时间： | 2006-05-22 |
Mutagenic Effects of BM302：GO112 Induced by Low-Energy Ion Beam Implantation |
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| ZHAO Shiguang, TANG Mingli , WANG Jun WANG Tao , WANG Shunehang , WU Yuejin YU Zengliang.Mutagenic Effects of BM302：GO112 Induced by Low-Energy Ion Beam Implantation[J].Plasma Science & Technology,2007,9(4):508-512. |
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| Authors: | ZHAO Shiguang TANG Mingli WANG Jun WANG Tao WANG Shunehang WU Yuejin YU Zengliang |
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| Affiliation: | 1 Key Laboratory of Ion Beam Bioengineering, Institute of Plasma Physics, Chinese Academy of Sciences, Hefei 230031, China |
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| Abstract: | mutagenic effects of ion beam implantation, BM302：GO112, physiological variation, L-sorbosone dehydrogenase, mutation |
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| Keywords: | mutagenic effects of ion beam implantation BM302：GO112 physiological variation L-sorbosone dehydrogenase mutation |
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