Affiliation: | 1. Translational Medicine Laboratory, and WIUCAS-FAHWMU Joint Center of Translational Medicine, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325035 China;2. Translational Medicine Laboratory, and WIUCAS-FAHWMU Joint Center of Translational Medicine, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325035 China
Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou, 325001 China
Oujiang Laboratory, Wenzhou, 325035 China |
Abstract: | Tissue-engineered scaffolds have been extensively explored for treating bone defects; however, slow and insufficient vascularization throughout the scaffolds remains a key challenge for further application. Herein, a versatile microfluidic 3D printing strategy to fabricate black phosphorus (BP) incorporated fibrous scaffolds with photothermal responsive channels for improving vascularization and bone regeneration is proposed. The thermal channeled scaffolds display reversible shrinkage and swelling behavior controlled by near-infrared irradiation, which facilitates the penetration of suspended cells into the scaffold channels and promotes the prevascularization. Furthermore, the embedded BP nanosheets exhibit intrinsic properties for in situ biomineralization and improve in vitro cell proliferation and osteogenic differentiation. Following transplantation in vivo, these channels also promote host vessel infiltration deep into the scaffolds and effectively accelerate the healing process of bone defects. Thus, it is believed that these near-infrared responsive channeled scaffolds are promising candidates for tissue/vascular ingrowth in diverse tissue engineering applications. |