| 葡萄糖-6-磷酸和果糖-6-磷酸的酶法测定 |
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| 引用本文： | 应汉杰,何永祥,顾海红,万红贵,欧阳平凯.葡萄糖-6-磷酸和果糖-6-磷酸的酶法测定[J].陶瓷科学与艺术,1997(1). |
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| 作者姓名： | 应汉杰 何永祥 顾海红 万红贵 欧阳平凯 |
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| 作者单位： | 南京化工大学生物科学与工科系 |
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| 摘 要： | 在血液、发酵液或组织液中加入磷酸葡萄糖异构酶（ＰＧＩ，ＥＣ．５．３．１．９）、６－磷酸葡萄糖脱氢酶（ＧＤＨ，ＥＣ．１．１．１．４．９）及还原型辅酶Ⅱ（β－ＮＡＤＰ），利用快速、准确的酶法检测Ｄ－６－磷酸葡萄糖和Ｄ－６－磷酸果糖浓度。对该法的专一性、精确性、准确性以及误差来源及消除方法作了讨论。对其它物质的分析作了探讨
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| 关 键 词： | 6－磷酸葡萄糖 6－磷酸果糖 酶法检测 |
ANALYSIS OF GLUCOSE 6 PHOSPHATE AND FRUCTOSE 6 PHOSPHATE BY ENZYMATIC METHOD |
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| Abstract: | A rapid and specific enzymic spectrophotometric method is described for the determination of D glucose 6 phosphate and D fructose 6 phosphate in deproteinized blood fermentation liquid and tissues by using phosphoglucose isomerase (PGI, EC.5.3.1.9) glucose 6 phosphate dehydrogenase (GDH, EC.1.1.1.49) and NADP, respectively. The optimum determination condition is defined. The method is specific, rapid and inexpensive. The specificity, precision, accuracy, and the sources of error and their elimination of this analysis method are discussed. |
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| Keywords: | glucose 6 phosphate\ fructose 6 phosphate\ enzymatic determination |
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