| 栗生灰黑孔菌合成漆酶最佳发酵条件的优化 |
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| 引用本文： | 李振光,沈明浩.栗生灰黑孔菌合成漆酶最佳发酵条件的优化[J].纺织学报,2011,32(12):64-70. |
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| 作者姓名： | 李振光 沈明浩 |
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| 作者单位： | 1. 吉林农业大学
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| 基金项目： | 吉林省科技厅课题(20090553) |
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| 摘 要： | 本实验研究了碳源、氮源、诱导剂、金属离子、pH等培养条件对栗生灰黑孔菌Melanoporia castanea (Imazeki)T. Hatt. & Ryvarden产漆酶的影响。并利用Minitab15软件、Plackett-Burman PB 实验设计和响应面分析法对栗生灰黑孔菌Melanoporia castanea (Imazeki)T. Hatt. & Ryvarden产漆酶的发酵条件进行优化。结果表明：1.最佳液态发酵培养基组分为：玉米秆粉9.976g/L，黄豆粉9g/L，ZnSO4?7H2O 0.3µmol /L，对苯二胺0.5mmol/L。2、最佳培养条件：温度28℃，转速180r/min，PH值为5.913，装液量60ml/250ml,接种量10%。在以上条件下培养漆酶酶活可达到431 U/ml。
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| 关 键 词： | 栗生灰黑孔菌 漆酶 培养条件 响应面 |
| 收稿时间： | 2010-12-01 |
Optimization of fermentation conditions of laccase of Melanoporia castanea |
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| LI Zhenguang,SHEN Minghao.Optimization of fermentation conditions of laccase of Melanoporia castanea[J].Journal of Textile Research,2011,32(12):64-70. |
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| Authors: | LI Zhenguang SHEN Minghao |
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| Affiliation: | LI Zhenguang,SHEN Minghao(Food Quality and Safety Laboratory Jilin Agricultural University,Changchun,Jilin 130118,China) |
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| Abstract: | The experiment studies the influence of culture conditions(carbon source,nitrogen source,revulsant,metallic ion,pH,temperature,rotation speed,the volume of liquid,inoculation amount of fungi) on the laccase production by Melanoporia castanea.Minitab15 software,and Plackett-Burman PB experimental design and response surface analysis were used to optimize the fermentation conditions of laccase of Melanoporia castanea.The results indicated that optimal liquid composition of fermentation medium:corn stalk powde... |
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| Keywords: | Melanoporia castanea laccase culture condition response surface |
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