| 转染SV40永生化基因正常成人肝细胞的形态观察 |
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| 引用本文： | 崔忻,姚文芳,高宇红,田磊,潘静坤,薛毅珑.转染SV40永生化基因正常成人肝细胞的形态观察[J].压电与声光,2010(4). |
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| 作者姓名： | 崔忻 姚文芳 高宇红 田磊 潘静坤 薛毅珑 |
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| 作者单位： | 解放军总医院老年医学研究所细胞生物学研究室; |
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| 基金项目： | 国家“863”计划项目(2006AA02Z498)~~ |
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| 摘 要： | 目的了解转染SV40基因对正常成人肝细胞形态结构及特殊染色的影响。方法采用本组构建的含SV40永生化基因的正常成人肝细胞(rSSR#69/HL-7702肝细胞),以HE染色法、糖原染色法(PAS)和葡萄糖-6-磷酸酶染色(G-6-Pase)法,在光镜下观察该细胞的形态特征,以电镜法观察该细胞的超微结构。结果:光镜下比较转染前、后的正常成人肝细胞形态无明显差异;PAS染色均可见胞浆内有粉红色的糖原颗粒,G-6-Pase染色均可见胞质内有铁黑色颗粒;电镜下比较转染前、后的正常肝细胞的超微结构亦无统计学差异。结论:转染SV40永生化基因的正常成人肝细胞的形态结构与转染前无明显差异,仍具有糖原合成及葡萄糖代谢的能力。
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| 关 键 词： | 永生化 肝细胞 形态 |
Morphology of SV40 transfection gene-immortalized liver cells in normal adults |
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| CUI Xin,YAO Wen-fang,GAO Yu-hong,TIAN Lei,PAN Jing-kun,XUE Yi-longCell Biology Laboratory.Morphology of SV40 transfection gene-immortalized liver cells in normal adults[J].Piezoelectrics & Acoustooptics,2010(4). |
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| Authors: | CUI Xin YAO Wen-fang GAO Yu-hong TIAN Lei PAN Jing-kun XUE Yi-longCell Biology Laboratory |
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| Affiliation: | CUI Xin,YAO Wen-fang,GAO Yu-hong,TIAN Lei,PAN Jing-kun,XUE Yi-longCell Biology Laboratory,Institute of Gerontology , Geriatries,Chinese PLA General Hospital,Beijing 100853,China |
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| Abstract: | Objective To study the effect of SV40 gene transfection on morphological structure of liver cells in normal adults and its special staining. Methods Liver cells (rSSR # 69/HL-7702 liver cells) containing SV40 gene from normal adults were stained with H&E, glycogen and glucose-6-phosphatase (G-6-Pase), and their morphological features and ultra-structure were observed under a light microscope and an electron microscope, respectively. Results No significant difference was found under light microscope in morph... |
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| Keywords: | Immortalized Hepatocytes Morphology |
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