High throughput synthesis and screening of new protein resistant surfaces for membrane filtration |
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| Authors: | Mingyan Zhou Hongwei Liu James E. Kilduff Robert Langer Daniel G. Anderson Georges Belfort |
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| Affiliation: | 1. Dept. of Civil and Environmental Engineering, Rensselaer Polytechnic Institute, Troy, NY 12180;2. Howard P. Isermann Dept. of Chemical and Biological Engineering, Rensselaer Polytechnic Institute, Troy, NY 12180;3. The Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180;4. Dept. of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139;5. David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139 |
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| Abstract: | A novel high throughput method for synthesis and screening of customized protein‐resistant surfaces was developed. This method is an inexpensive, fast, reproducible and scalable approach to synthesize and screen protein‐resistance surfaces appropriate for a specific feed. The method is illustrated here by combining a high throughput platform (HTP) approach together with our patented photo‐induced graft polymerization (PGP) method developed for facile modification of commercial poly(aryl sulfone) membranes. This new HTP‐PGP method was validated by comparison with our previous published results obtained using a bench‐scale filtration assay of six well‐studied monomers. Optimally‐performing surfaces for resisting a model protein, bovine serum albumin (BSA), were identified from a library of 66 monomers. Surfaces were prepared via graft polymerization onto poly(ether sulfone) (PES) membranes and were evaluated using a protein adsorption assay followed by pressure‐driven filtration. Bench‐scale verification was conducted for selected monomers using HTP‐PGP method; a good correlation with HTP‐PGP results was found. © 2009 American Institute of Chemical Engineers AIChE J, 2010 |
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| Keywords: | high throughput protein‐resistant surfaces photo‐induced graft polymerization bovine serum albumin membrane filtration |
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