用化学发光法分析燕麦发芽前后酚类物质抗氧化活性

对燕麦种子进行发芽实验6 d,对原样、6 d籽粒、6 d芽和6 d根中的酚类物质进行提取纯化,采用化学发光法对不同部位纯化物清除活性氧自由基的能力进行测定。结果表明:原样、6 d籽粒、6 d芽和6 d根纯化物在不同体系中均表现出良好的抗氧化活性,其清除超氧阴离子的IC50值分别为0.952、0.702、3.248、1.060 mg/mL,清除羟基自由基的IC50值分别为8.80、3.31、13.69、11.68μg/mL,清除双氧水的IC50值分别为10.97、8.64、13.05、9.61μg/mL,可见发芽过程使燕麦籽粒在3个体系中的抗氧化活性均有所提高,而燕麦芽的抗氧化活性相对籽粒和根较差。

The Comparison of Antioxidant Activity of Phenols in Oat After Germination by Chemiluminescence Method

Oat seeds were soaked and germinated.Phenol compounds from non-germination oat,the grain of 6d,the bud of 6d and the root of 6d were extracted and purified.Antioxidant activities of purified products from different parts were studied.The results indicated that the purified products from non-germination oat,the grain of 6d,the bud of 6d and the root of 6d showed strong radical scavenging activity in various antioxidant assays.The IC50 of O-2· scavenging effect were 0.952 mg/mL,0.702mg/mL,3.248mg/mL,1.060mg/mL respectively.The IC50 of ·OH scavenging effect were 8.80μg/mL,3.31μg/mL,13.69μg/mL,11.68·g/mL respectively.The IC50 of H2O2 scavenging effect were 10.97μg/mL,8.64μg/mL,13.05μg/mL,9.61μg/mL respectively.The antioxidant activity of oat grain was increased to a certain extent during oat germination,while the antioxidant activity of oat bud was lower than oat grain and root.