| 蛋清溶菌酶的提取分离与酶活研究 |
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| 引用本文： | 吴旭亚,游庆红,尹秀莲. 蛋清溶菌酶的提取分离与酶活研究[J]. 广州化工, 2012, 40(19): 51-53 |
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| 作者姓名： | 吴旭亚 游庆红 尹秀莲 |
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| 作者单位： | 1. 江苏柯菲平医药有限公司,江苏南京,210016
2. 淮阴工学院生命科学与化学工程学院,江苏淮安,223001 |
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| 基金项目： | 潍安市科技支撑项目（No：SN1160）; 江苏省生物质能与酶技术重点实验室开放课题（No：JSBEET1203） |
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| 摘 要： | 以鸡蛋清为原料,采用正交实验分别确定盐析法与离子交换法两种提取方法的最佳条件,同时采用比浊法测定酶活力。确定了盐析法从蛋清中提取溶菌酶的最佳条件是：加入蛋清滤液体积5%的氯化钠;结晶的温度4℃;结晶的时间为4天;离子交换法的最佳吸附条件为：蛋清树脂比5∶1,pH 7.0,搅拌吸附时间为4 h。离子交换法所得酶的产量与活力均高于盐析法。
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| 关 键 词： | 溶菌酶 蛋清 盐析法 离子交换法 |
Optimum Extraction Technology of Egg White Lysozyme and Determination of Enzyme Activity |
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| WU Xu-ya,YOU Qing-hong,YIN Xiu-lian. Optimum Extraction Technology of Egg White Lysozyme and Determination of Enzyme Activity[J]. GuangZhou Chemical Industry and Technology, 2012, 40(19): 51-53 |
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| Authors: | WU Xu-ya YOU Qing-hong YIN Xiu-lian |
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| Affiliation: | 1 Jiangsu Carefree Pharmaceutical Co.,Ltd.,Jiangsu Nanjing 223001;2 College of Life Sciences and Chemical Engineering,Huaiyin Institute of Technology,Jiangsu Huai＇an 223001,China) |
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| Abstract: | Using egg white as material,enzyme activity as target,the alting-out method and ion exchange extraction methods were studied by orthogonal experiment design.The optimal process conditions for extraction lysozyme were adding 5% sodium chloride,crystallization temperature 4 ℃,and crystallization time for 4 days.The best conditions of Ion exchange were egg white resin ratio 5：1,pH 7.0,and stirred adsorption time for 4 h.The output and enzyme activity of enzyme derived from ion exchange was higher than that got from the salting-out method. |
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| Keywords: | lysozyme egg white salt-out method ion exchange |
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