应用单细胞微凝胶电泳技术检测成纤维细胞的放射敏感性

应用单细胞微凝胶电泳（SCGE）技术以及噻唑蓝（MTT）比色法检测辐射诱发的成纤维细胞系AT5BIVA和GM637的初始DNA双链断裂数及断裂拍的修复与细胞放射敏感性之间的关系。结果表明，AT5BIVA细胞系的放射敏感性显著高于GM637。两种细胞系的初始DNA双链断裂数均随剂量的增加而增加，呈显著的剂量效应关系，在给定的剂量点，辐射诱发的AT5BIVA细胞系的初始DNA双链断裂数显著高于GM637。AT5BIVA纱对辐射诱发的DNA双链断裂的修复能力小于GM637。结果提示，辐射诱发的初始DNA双链断裂数及断裂后的修复与细胞的放射敏感性均有一定的相关性，作为细胞放射敏感性预测指标具有很大的应用潜势。

USE OF SINGLE CELL MICROGEL ELECTROPHORESIS TO DETECT THE CELLUAR RADIOSENSITIVITY OF TWO FIBROBLAST STRAINS

Single cell microgel electrophoresis(SCGE) and MTT assay were used to determine the relationships between radiation-induced initial DNA double-strand breaks(DSBs), the ability of cells to repair DSBs and cellular radiosensitivity in both AT5BIVA and GM637 cell lines. The results demonstrated that AT5BIVA cell line was significantly more radiosensitive than GM637 . The initial DSBs in both AT5BIVA and GM637 lines increased with the rise of irradiation dose, and showed significant dependence on dose. At a given dose of 20Gy, radiation-induced initial DSBs in AT5BIVA cell line was signficantly higher than those in GM637. The ability of AT5BIVA cell line to repair DSBs was more powerful than that of GM637 . The results suggested that the radiation-induced initial DSBs in cells and the ability of cells to repair DSBs both correlated with cellular radiosensitivity to a certain extent, and could be used as potential predictors of intrinsic radiosensitivity of cells.