响应面法优化甘露醇产丁醇的发酵条件

以褐藻的主要成分甘露醇为底物，利用丙酮丁醇梭菌ATCC824发酵生产丁醇。采用Plackett-Burman（P-B）和Box-Behnken优化发酵条件。在Plackett-Burman实验设计时，考察牛肉膏、酵母浸粉、胰蛋白胨、乙酸铵、 KH2PO4、MgSO4?7H2O、FeSO4?7H2O、接种量、发酵温度、初始pH值这10个因素对丁醇产量影响，筛选出影响丁醇发酵的重要参数是发酵温度、初始pH值、乙酸铵浓度，利用Box-Behnken设计确定最优发酵条件为发酵温度37.3 ℃、初始pH值6.38、乙酸铵浓度2.82 g/L。数学模型分析预测最大丁醇产量为8.47 g/L。经实验验证表明，在优化条件下的丁醇产量平均值达到8.52±0.55 g/L。这说明利用统计学方法优化甘露醇的丁醇发酵条件是有效的。

Optimization of butanol production from mannitol by Clostridium acetobutylicum ATCC824 using response surface method

Mannitol，which is the main component of brown algae，was utilized in this study to produce butanol by Clostridium acetobutylicum (ATCC 824). Plackett-Burman (P-B) and Box-Behnken designs were adopted to optimize the fermentation conditions. Ten factors，including beef extract，yeast extract，tryptone，ammonium acetate，KH2PO4，MgSO4?7H2O，FeSO4?7H2O，size of inoculum，fermentation temperature and initial pH，were considered to affect butanol production in the Plackett-Burman (P-B) design. The result demonstrated that fermentation temperature，initial pH and concentration of ammonium acetate were the most critical factors，and optimal fermentation conditions were 37.3℃，pH 6.38 and 2.82 g/L of ammonium acetate according to the Box-Behnken design. A mathematical model was established and used to predict the maximum butanol yield of 8.47 g/L. The result of verification experiment showed that the maximum butanol yield reached 8.52±0.55g/L under the optimum conditions，which showed that the statistical method was a useful tool for butanol production from mannitol in the optimization.