用单壁纳米碳管制备葡萄糖生物传感器的研究

以单壁纳米碳管为代表材料,对利用纳米碳管制备葡萄糖生物传感器中纳米碳管的作用和纳米碳管修饰电极的方法、酶的固定化方法及电极种类等因素对传感器性能的影响进行了研究.研究结果表明,纳米碳管的加入能有效地改善传感器的电化学性能,利用二茂铁和单壁纳米碳管共同修饰电极所制得的传感器的性能要好于仅用单壁纳米碳管修饰电极制得的传感器.在酶的固定化方法中,戊二醛交联法要略好于明胶包埋法;而利用铂电极制备出的生物传感器对葡萄糖的响应电流要明显高于利用金电极和玻碳电极制备出的生物传感器.这些结论对于开发纳米碳管在生物传感领域及生命科学相关领域的应用有参考价值.

Researches on preparing glucose biosensor by single-walled carbon nanotubes

Taking single-walled carbon nanotubes (SWNTs) as a basic material, the paper studies the preparation of glucose biosensors with carbon nanotubes (CNTs) . Specifically, it studies the effect of CNTs in the biosensor' s fabrication and the factors in influencing the biosensor's performance such as electrode surface modification by CNTs, enzyme immobilization, and categories of electrode during the preparation of glucose biosensor by CNTs. Results show that the input of CNTs can improve the electrochemical property of sensor effectively, and the property of biosensor prepared on the electrode co-modified by ferrocene and SWNTs is better than that prepared on the electrode only modified by SWNTs. In immobilization methods of enzyme, glutaraldehyde cross-linkage is superior to gelatine embedding in some degree, and the response current to glucose of biosensor prepared on Pt electrode is much higher than biosensors prepared on Au and glassy carbon (GC) electrodes.