| Lutein and lutein esters in marigold flowers by high performance chromatography |
| |
| 作者姓名： | 蒋新宇 陈龙胜 周春山 |
| |
| 作者单位： | SchoolofChemistryandChemicalEngineering,CentralSouthUniversity,Changsha410083,China |
| |
| 基金项目： | Project(00KNY1010)supportedbytheKeyProgramofScienceandTechnologyOfficeofHunanProvince |
| |
| 摘 要： | Lutein and lutein esters in marigold flowers was quantitatively determined by high performance chromatography (HPLC) with ODS-C18 column. A mixture of CH3CN-CH3OH-CH3COOCH2CH2 with volume ratio of 55:1:44 was used as mobile phase at a flow rate of 1.0mL/min and detection was carried out at 480 nm. The column temperature was about 20℃. The contents of lutein and lutein esters were determined by analytical curve of lutein since lutein and lutein esters have the same spectral characteristics. Determination results of hexane extracts and saponified samples of lutein show that the saponification transforms the esters into free lutein. The increase of the content of dipalmitate and palmitate stearate reveals that the reaction includes transesterifications.
|
| 关 键 词： | HPLC 叶黄素 酯 金盏花 |
| 收稿时间： | 9 June 2004 |
| 修稿时间： | 26 October 2004 |
Lutein and lutein esters in marigold flowers by high performance chromatography |
| |
| Jiang Xin-yu , Chen Long-shen and Zhou Chun-shan.Lutein and lutein esters in marigold flowers by high performance chromatography[J].Journal of Central South University of Technology,2005,12(3):306-308. |
| |
| Authors: | Jiang Xin-yu Chen Long-shen and Zhou Chun-shan |
| |
| Affiliation: | (1) School of Chemistry and Chemical Engineering, Central South University, 410083 Changsha, China |
| |
| Abstract: | Lutein and lutein esters in marigold flowers was quantitatively determined by high performance chromatography (HPLC) with ODS-C18 column. A mixture of CH3CN - CH3OH - CH3COOCH2CH3 with volume ratio of 55: 1: 44 was used as mobile phase at a flow rate of 1.0 mL/min and detection was carried out at 460 nm. The column temperature was about 20 ℃. The contents of lutein and lutein esters were determined by analytical curve of lutein since lutein and lutein esters have the same spectral characteristics. Determination results of hexane extracts and saponified samples of lutein show that the saponification transforms the esters into free lutein. The increase of the content of dipalmitate and palmitate stearate reveals that the reaction includes transesterifications. |
| |
| Keywords: | HPLC lutein lutein ester marigold flower |
| 本文献已被 CNKI 维普 万方数据 SpringerLink 等数据库收录！ |
