| 应用核酸探针检测甲型肝炎疫苗的滴度 |
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| 引用本文： | 李琦涵,袁天喜,曹逸云,董德祥,戴长柏,苏晔.应用核酸探针检测甲型肝炎疫苗的滴度[J].粉末涂料与涂装,1992(2). |
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| 作者姓名： | 李琦涵 袁天喜 曹逸云 董德祥 戴长柏 苏晔 |
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| 作者单位： | 中国医学科学院医学生物学研究所 昆明650107 |
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| 摘 要： | 应用甲型肝炎病毒(HAV)cDNA 片段作为探针,分别以同位素、异羟基洋地黄毒苷配基和生物素标记该探针,对甲肝疫苗病毒培养过程中不同时期、不同接种量的病毒浓度进行了滴定,并以 ELISA 作对照。结果表明,各种标记探针检测的灵敏度均高于 ELISA。而不同标记的探针检测的结果无显著差异。
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| 关 键 词： | cDNA探针 标记物 疫苗毒力滴定 |
Detection of HAV Vaccine Titre with the Nucleic Acid Probe |
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| Li Qihan Institute of Medical Biology,CAMS,Kunming.Detection of HAV Vaccine Titre with the Nucleic Acid Probe[J].Chinese Journal of Biologicals,1992(2). |
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| Authors: | Li Qihan Institute of Medical Biology CAMS Kunming |
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| Abstract: | During the course of cultivation of live attenuated HAV,the virus titres were detectedat different time and with various quantities of inoculum by using nucleic acid probes labelled with iso-tope,digoxigenin and biotin respectively,and compared with ELISA.The results showed that the sen-sitivity of the probes were higher than that of ELISA.There was no any obvious difference amongthree kinds of probes. |
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| Keywords: | cDNA probe Detection of HAV vaccine titre |
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