Radio-resistance induced by nitric oxide to heavy ion irradiation in A172 human glioma cells |
| |
| Authors: | ZHOU Qingming ZHANG Hong ZHANG Xingxia |
| |
| Affiliation: | 1. Center of Cardiology I, Cardiology I, University Medical Center Mainz at the Johannes Gutenberg University, Germany;2. First Department of Medicine, University Medical Center Mainz at the Johannes Gutenberg University, Germany;1. Center for Biophysics & Department of Theoretical Physics, Saarland University, Saarbrücken 66041, Germany;2. Department of Molecular Biophysics, Center for Integrative Physiology and Molecular Medicine, Saarland University, Homburg 66421, Germany;3. Department of Biophysics, Center for Integrative Physiology and Molecular Medicine, Saarland University, Homburg 66421, Germany;4. Molecular Physiology, Institute of Cardiovascular Physiology, University Medical Center Georg-August-University, Göttingen 37073, Germany |
| |
| Abstract: | ![]()
To investigate effects of nitric oxide on cellular radio-sensitivity, three human glioma cell lines, i.e. A172,A172 transfected green fluorescence protein (EGFP) gene (EA172) and A172 transfected inducible nitric oxide synthesis (iNOS) gene (iA172), were irradiated by 12C6 ions to 0, 1 or 2Gy. Productions of nitric oxide and glutathione (GSH) in A172, EA172 and iA172 were determined by chemical methods, cell cycle was analyzed by flow cytometry at the 24th hour after irradiation, and survival fraction of the cells was measured by colorimetric MTT assay at the 5th day after irradiation. The results showed that the concentrations of nitric oxide and GSH in iA172 were significantly higher than in A172 and EA172; the G2/M stage arrest induced by the 12C6 ion irradiation was observed in A172 and EA172 but not in iA172 at the 24th hour after exposure; and the survival fraction of iA172 was higher than that of EA172 and iA172. Data suggest that the radio-sensitivity of the A172 was reduced after the iNOS gene transfection.The increase of GSH production and the change of cellular signals such as the cell cycle control induced by nitric oxide may be involved in this radio-resistance. |
| |
| Keywords: | Nitric oxide 12C 6 heavy ion A172 human glioma cells Radio-resistance glioma cells human heavy ion irradiation nitric oxide increase production signals cell cycle control Data gene transfection exposure stage arrest induced results concentrations survival fraction MTT assay flow cytometry |
| 本文献已被 CNKI 维普 万方数据 ScienceDirect 等数据库收录！ |
|
