| 紫外线和硫酸二乙酯诱变高产凝乳酶地衣芽孢杆菌的研究 |
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| 引用本文： | 张卫兵,梁琪,乔海军,米兰,张炎,杨敏,甘伯中.紫外线和硫酸二乙酯诱变高产凝乳酶地衣芽孢杆菌的研究[J].食品工业科技,2012,33(5):174-176,184. |
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| 作者姓名： | 张卫兵 梁琪 乔海军 米兰 张炎 杨敏 甘伯中 |
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| 作者单位： | 甘肃农业大学食品科学与工程学院,甘肃省功能乳品工程实验室,甘肃省干酪素工程技术研究中心,甘肃兰州730070 |
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| 基金项目： | 国家863计划(2011AA100903);甘肃省科技重大专项(0702NKDA034);甘肃农业大学校创新基金(GAU-CX1107) |
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| 摘 要： | 以产凝乳酶地衣芽孢杆菌为出发菌株,通过紫外线诱变和硫酸二乙酯诱变,提高菌株的凝乳活力。经反复诱变筛选获得一株凝乳活力较高且水解活力较低的突变株DES-6,其凝乳活力为184.65SU/mL,比原菌株增加了15.41%,水解活力为23.35U/mL,比原菌株降低了64.80%。传代实验表明,突变株DES-6具有稳定的遗传性。
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| 关 键 词： | 凝乳酶 地衣芽孢杆菌 诱变 |
Study on mutation breeding of Bacillus licheniformis producing chymosin by ultra violet and diethyl sulfate |
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| ZHANG Wei-bing,LIANG Qi,QIAO Hai-jun,MI Lan,ZHANG Yan,YANG Min,GAN Bo-zhong.Study on mutation breeding of Bacillus licheniformis producing chymosin by ultra violet and diethyl sulfate[J].Science and Technology of Food Industry,2012,33(5):174-176,184. |
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| Authors: | ZHANG Wei-bing LIANG Qi QIAO Hai-jun MI Lan ZHANG Yan YANG Min GAN Bo-zhong |
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| Affiliation: | *(College of Food Science and Engineering,Gansu Provincial Key Laboratory of Functional Dairy,Gansu Casein Engineering Technical Research Centre,Lanzhou 730070,China) |
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| Abstract: | Bacillus licheniformis producing chymosin was mutated by ultraviolet and diethyl sulfate to improve the ability of producing chymosin.After screening repeatedly,a mutant DES-6 with high activity of chymosin and lower proteolysis was obtained.Compared with the original strain,the activity of chymosin increased by 15.41%,the proteolytic activity reduced by 64.80%.DES-6 had good hereditary stability after several generations. |
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| Keywords: | chymosin Bacillus licheniformis mutation |
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