| 水相体系酶法制备甘油磷脂酰胆碱的研究 |
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| 引用本文： | 张康逸,陈岩,刘元法,王兴国.水相体系酶法制备甘油磷脂酰胆碱的研究[J].食品工业科技,2012,33(10):219-222. |
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| 作者姓名： | 张康逸 陈岩 刘元法 王兴国 |
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| 作者单位： | 江南大学食品学院,食品科学与技术国家重点实验室,江苏无锡214122 |
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| 基金项目： | 国家863高技术研究发展计划(2010AA101506) |
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| 摘 要： | 研究了脂肪酶(Thermo4S-3)在水相体系中制备甘油磷脂酰胆碱(GPC)的可行性,并探讨了脂肪酶水解条件对GPC得率的影响。实验结果表明脂肪酶(Thermo4S-3)可以实现豆粉末磷脂的水解反应,具有制备甘油磷酸胆碱(GPC)的工业应用前景。正交实验优化结果如下:反应温度40℃,底物浓度33.3g/L,酶添加量30U/mL,CaCl2添加量2g/L。利用HPLC-ELSD进行定量分析,GPC得率达到96.5%。LC-MS/MS定性分析,GPC的结构与标样分析的一致。
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| 关 键 词： | 磷脂酰胆碱 甘油磷脂酰胆碱 脂肪酶(Thermo4S-3) 酶解反应 |
Preparation of glyceryl phosphoryl choline catalyzed by Thermo4S-3 |
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| ZHANG Kang-yi,CHEN Yan,LIU Yuan-fa,WANG Xing-guo.Preparation of glyceryl phosphoryl choline catalyzed by Thermo4S-3[J].Science and Technology of Food Industry,2012,33(10):219-222. |
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| Authors: | ZHANG Kang-yi CHEN Yan LIU Yuan-fa WANG Xing-guo |
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| Affiliation: | (State Key Laboratory of Food Science and Technology,School of Food Science and Technology,Jiangnan University,Wuxi 214122,China) |
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| Abstract: | Thermo4S-3 was applied to prepare of glyceryl phosphoryl choline(GPC) in aqueous media.The effects of reaction parameters on the yield of GPC were discussed.The optimum conditions were as follows:Temperature 40℃,substrate concentration 33.3g/L,Thermo4S-3 loading 30U/mL,dosage of CaCl2 2g/L.The final GPC was determined by HPLC-ELSD with the yield 96.5%.The results of LC-MS/MS showed that the structure of L-α-GPC was agreement with that of standard sample.The results indicated that our study provided a feasible method for synthesis of GPC. |
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| Keywords: | phosphatidyl choline glyceryl phosphoryl choline lipase(Thermo4S-3) enzymatic reaction |
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