| 进出口动物源性食品中志贺氏菌检验方法优化 |
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| 引用本文： | 郑海松,李刚,谢科,余晓峰,孙娟娟,李云飞,陈雪娇.进出口动物源性食品中志贺氏菌检验方法优化[J].食品工业科技,2012,33(6):108-110,119. |
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| 作者姓名： | 郑海松 李刚 谢科 余晓峰 孙娟娟 李云飞 陈雪娇 |
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| 作者单位： | 1. 安徽出入境检疫检验局,安徽合肥,230022
2. 合肥工业大学生物与食品工程学院,安徽合肥,230009 |
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| 基金项目： | 国家标准项目(20073670-T-424);出入境检验检疫行业标准项目(2009B165) |
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| 摘 要： | 为建立进出口动物源性食品中志贺氏菌检验方法,从检测条件入手,通过增菌方式、分离培养基特异性和生化鉴定优化方法,以不同种类食品检测和不同实验室检测验证方法。确定的检测条件为志贺氏菌增菌液中(41.5±1)℃厌氧培养16～20h作为志贺氏菌检测的增菌方式;在分离上,同时使用MAC、XLD和HE三种平板,以提高志贺氏菌的检出率;选择了微量生化管和API20E对志贺氏菌和其他肠杆菌进行了生化鉴定和比较,效果明显。以此方法检测人工污染食品均检出志贺氏菌,各实验室也均检出志贺氏菌。
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| 关 键 词： | 志贺氏菌 动物源性食品 增菌 分离培养基 生化鉴定 |
Optimization of shigellae inspection method for import and export animal-derived food |
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| Affiliation: | ZHENG Hai - song1,LI Gang1,XIE Ke2,YU Xiao - feng1,SUN Juan - juan1,LI Yun - fei1,CHEN Xue - jiao1 (1. Anhui Entry - Exit Inspection and Quarantine Bureau,Hefei 230022,China; 2. Academy of Biotechnology and Food Engineering,Hefei University of Technology,Hefei 230009,China) |
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| Abstract: | In order to establish the inspection method of shigellae in animal - derived foods for import and export,the detection conditions were optimized in the enrichment culture,specificity of separation medium and biochemical identification,the method were verified by the detection for different kinds of food and different laboratories. This detection conditions confirmed showed that the best enrichment culture method of shigellae was(41. 5±1)℃ anaerobic training for 16 ~ 20h. In separation,MAC,XLD and HE were used at the same time to improve the detectable rate of shigellae infection. There were significant effects between the trace of biochemical tube and API20E in identification and comparison shigellae and other enterbacteriaceae. With the method,shigellae were all detected in artificially contaminated food by each laboratory. |
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| Keywords: | shigellae animal - derived food enrichment culture separation medium biochemical identification |
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