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| 大分子葡聚糖-牛血清蛋白抗原偶联物的制备与表征 | |
| 引用本文： | 方丽莎,谢珍茗,梁达奉,余林. 大分子葡聚糖-牛血清蛋白抗原偶联物的制备与表征[J]. 食品工业科技, 2010, 0(3) |
| 作者姓名： | 方丽莎 谢珍茗 梁达奉 余林 |
| 作者单位： | 1. 广东工业大学轻工化工学院,广东,广州,510003 2. 广州蔗糖研究所,广东,广州,510316 |
| 摘 要： | 用还原胺化法制备大分子葡聚糖-牛血清蛋白(BSA)拟糖蛋白抗原,通过SDS-聚丙烯酰胺凝胶电泳结合考马斯亮蓝和过碘酸-品红两种染色方法验证偶联物的生成。考察不同反应条件(葡聚糖T40的氧化度,偶联pH,氧化葡聚糖和蛋白质的比值,偶联时间)对偶联反应的影响。确定拟糖蛋白抗原的最佳偶联条件为:葡聚糖T40/氧化剂NaIO4=1/120(物质的量比),偶联溶液pH8.0,葡聚糖T40/BSA=1/1(物质的量比),偶联时间24h。并将此反应条件用于T10和T100拟糖蛋白抗原的合成。 |
| 关 键 词： | 葡聚糖 拟糖蛋白 抗原 偶联条件 |
Synthesis and characterization of macromolecular dextran and protein conjugates antigen |
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| FANG Li-sha,XIE Zhen-ming,LIANG Da-feng,YU Lin. Synthesis and characterization of macromolecular dextran and protein conjugates antigen[J]. Science and Technology of Food Industry, 2010, 0(3) | |
| Authors: | FANG Li-sha XIE Zhen-ming LIANG Da-feng YU Lin |
| Affiliation: | FANG Li-sha1,XIE Zhen-ming1,LIANG Da-feng2,YU Lin1,(1.Faculty of Light , Chemical,Guangdong University of Technology,Guangzhou 510003,China,2.Guangzhou Institute of Sucrose,Guangzhou 510316,China) |
| Abstract: | A high active yield dextran-BSA conjugate antigen was prepared by Reductive Amination Method.The covalent attachment of dextran to protein was confirmed by SDS- PAGE stained Coomassie brilliant blue and PAS-fuchsin. Different reaction conditions including oxidation of dextran, pH, dextran- and BSA concentrations, reaction time various,showed different effects on the entire reaction.The optimum condition was dextran/NalO_4=1: 120, pH 8.0, dextran/BSA = 1 : 1 and 24h.And this reaction conditions to be used for synthesized other neoglycoproteins antigens contained T10 and T100. |
| Keywords: | dextran neoglycoprotein antigen reaction conditions |
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