Distribution of penicillin in the cornea, tears and aqueous humour at various times following a single intravenous injection

Bioassays performed by the disc diffusion technique on samples of blood, tears, aqueous humour and cornea from 30 rabbits killed 4 to 24 hours following a single intravenous injection of benzylpenicillin (40 mg/kg) showed that the penicillin content of these fluids and tissues progressively decreased with time. However, even after 24 hours the agent could be detected in the samples. The peripheral and central cornea contained similar amounts of penicillin up to 16 hours; thereafter, the peripheral cornea contained more of the agent.