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Simple procedure for fatty acid analysis of glycerophospholipids in Escherichia coli and Saccharomyces cerevisiae |
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| Authors: | Ichihara Ken'ichi Tani Atsumi |
| Affiliation: | Department of Biomolecular Chemistry, Kyoto Prefectural University, Shimogamo, Kyoto 606-8522, Japan; Bio-Analysis Center, Advanced Scientific Technology & Management Research Institute of Kyoto, Chudoji, Kyoto 600-8813, Japan. |
| Abstract: | Rapid, convenient methods have been developed for fatty acid analysis of membrane glycerophospholipids in microorganisms. Fatty acid methyl esters derived from glycerophospholipids have been prepared directly from wet pellets of Escherichia coli cells or Saccharomyces cerevisiae spheroplasts without lipid extraction and fractionation in high yields under mild temperature conditions for analysis by gas chromatography. |
| Keywords: | Fatty acid analysis Fatty acid methyl ester Phospholipid Gas chromatography Escherichia coli Saccharomyces cerevisiae |
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