| 小麦热激蛋白基因sHSP16.9植物表达载体构建 |
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| 引用本文： | 李静婷,牛磊,侯玉杰. 小麦热激蛋白基因sHSP16.9植物表达载体构建[J]. 河南城建高等专科学校学报, 2013, 0(6): 76-79 |
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| 作者姓名： | 李静婷 牛磊 侯玉杰 |
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| 作者单位： | [1] 平顶山学院资源与环境科学学院,河南平顶山467000 [2] 河南城建学院测绘工程学院,河南平顶山467036 [3] 河南城建学院生物科学与工程学院,河南平顶山467036 |
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| 基金项目： | 河南省教育厅科技攻关计划项目（128180020）. |
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| 摘 要： | 以小麦中国春热激蛋白基因sHSP1 6.9为材料,用PCR扩增的方法于目的基因片段5和3上分别添加Xba Ⅰ和Kpn Ⅰ的酶切位点,然后与植物表达载体pCAMBIA Super-1300连接.用Xba Ⅰ和Kpn Ⅰ进行双酶切验证,确定已将热激蛋白基因sHSP16.9连接到植物表达载体上,表达载体pCAMBIA Super-1300-TasHSP16.9构建成功.为验证小麦热激蛋白基因sHSP16.9的功能及转基因植物表达奠定基础.
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| 关 键 词： | 小麦 热激蛋白 植物表达载体 载体构建 |
On plant expression vector of heat shock protein gene sHSP16.9 in wheat |
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| TT Jing-tlng,NIU Lei,HOU Yu-jie. On plant expression vector of heat shock protein gene sHSP16.9 in wheat[J]. Journal of Henan Urban Construction Junior College, 2013, 0(6): 76-79 |
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| Authors: | TT Jing-tlng NIU Lei HOU Yu-jie |
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| Affiliation: | 3 ( 1. College of Resources and Environmental Science, Pingdingshan University, Pingdingshan 467000, China ; 2. School of Surveying Engineering, Henan University of Urban Construction, Pingdingshan 467036, China ; 3. School of Biological Science ＆ Engineering, Henan University of Urban Construction, Pingdingshan 467036, China) |
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| Abstract: | Taking Chinese Spring wheat heat shock protein gene sHSP16.9 as materials, using methods for PCR amplification to add Xba I and Kpn I to restriction sites of target gene fragments 5 and 3, and then con- nect the plant expression vector pCAMBIA Super-1300. And double digestion validation is conducted with Xba I and Kpn I to confirm that the heat shock protein genes have been connected to sHSP16.9 plant expression vector, and the expression vector pCAMBIA Super-1300-TasHSP16.9 constructed successfully, which lays foundation for verifying the functions of wheat heat shock protein gene sHSP16.9 and transgenic plant expres- sion. |
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| Keywords: | wheat heat shock protein plant expression vector vector construction |
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