| 磁性壳聚糖微球固定化脂肪酶的研究 |
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| 引用本文： | 常凯,王红英,孙井辉,钱斯日古楞.磁性壳聚糖微球固定化脂肪酶的研究[J].大连工业大学学报,2011,30(1):30-33. |
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| 作者姓名： | 常凯 王红英 孙井辉 钱斯日古楞 |
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| 作者单位： | 大连工业大学生物工程学院,辽宁大连,116034 |
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| 摘 要： | 以纳米级磁性壳聚糖微球为载体,戊二醛为交联剂固定化脂肪酶,对固定化脂肪酶条件进行优化,同时对固定化脂肪酶的理化性质、活性回收率、热稳定性和储存稳定性进行研究.结果表明,固定化脂肪酶的最佳时间为7 h,最佳pH为8,最佳温度为50 ℃,最佳酶加量为每100 mg磁性微球加酶10 mg.和自由酶相比,脂肪酶被磁性壳聚糖固定...
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| 关 键 词： | 磁性微球 固定化酶 脂肪酶 |
Immobilization of lipase onto magnetic chitosan microspheres |
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| CHANG Kai,WANG Hong-ying,SUN Jing-hui,QIAN Si-riguleng.Immobilization of lipase onto magnetic chitosan microspheres[J].Journal of Dalian Dalian Polytechnic University,2011,30(1):30-33. |
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| Authors: | CHANG Kai WANG Hong-ying SUN Jing-hui QIAN Si-riguleng |
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| Affiliation: | CHANG Kai,WANG Hong-ying,SUN Jing-hui,QIAN Si-riguleng School of Bioengineering,Dalian Polytechnic University,Dalian 116034,China |
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| Abstract: | The immobilized condition of lipase onto magnetic chitosan microspheres by cross-linking with glutaldehyde was optimized and the immobilized lipase was characterized.The optimum immobilizing time was 7 h,the optimum pH was 8,the optimum temperature was 50 ℃,and the optimum lipase amount were added 10 mg in 100 mg microspheres.Compared with free lipase,the thermal and acid-basic stabilities of immobilized lipase were increased appreciably.After 5 recycle of hydrolyzation,the activity of immobilized lipase remained 82.6% of its initial activity.Magnetic chitosan microsphere was a kind of good support for immobilizing lipase. |
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| Keywords: | magnetic microspheres immobilized enzyme lipase |
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