| 循环流液相等电聚焦方法及应用研究 |
| |
| 引用本文： | 顾增旺,邵晓霞,张丽华,屠红旻,夏其昌.循环流液相等电聚焦方法及应用研究[J].高技术通讯,1994(2). |
| |
| 作者姓名： | 顾增旺 邵晓霞 张丽华 屠红旻 夏其昌 |
| |
| 作者单位： | 中国科学院上海生物化学研究所 |
| |
| 摘 要： | 利用循环流液相等电聚焦技术，对Ｈｂ、Ｃｙｔ．Ｃ和ＢＳＡ混合物进行分离，对ＲＮａｓｅＡ粗制品进行精制。该方法上样量大，活性回收好。对聚焦过程中扩散、对流和电渗的影响进行了讨论。
|
| 关 键 词： | 循环流液相等电聚焦，制备型蛋白分离 |
Recycling Free-Flow Isoelectric Focusing and Its Application |
| |
| Gu Zengwang, Shao Xiaoxia, Zhang Lihua, Tu Hongmin, Xia Qichang.Recycling Free-Flow Isoelectric Focusing and Its Application[J].High Technology Letters,1994(2). |
| |
| Authors: | Gu Zengwang Shao Xiaoxia Zhang Lihua Tu Hongmin Xia Qichang |
| |
| Affiliation: | (received Oct. 15,1993,revised Nov. 24)Gu Zengwang, Shao Xiaoxia, Zhang Lihua, Tu Hongmin, Xia Qichang(Shanghai Institute of Biochemistry,Academia Sinica,Shanghai 200031 ) |
| |
| Abstract: | new method for preparative isoelectric focusing, recycling free-flow isoelectric focusing (RIEF),was described. It was used to separate a mixture of model protein (hemoglobin bovine serum albumin,cytochrome c). RNase A was also purified from crude RNase by RIEF. High protein loading and highrecovery of activity were achieved. Some harmful effects in IEF, such as diffusion, convection andelectroosmosis, were discussed. |
| |
| Keywords: | : Recycling free-flow isoelectric focusing Preparative separation of protein |
| 本文献已被 CNKI 等数据库收录！ |
|
