马兰总黄酮提取工艺优化及不同部位含量测定

以马兰全株为材料运用超声波辅助法进行黄酮类化合物的提取,采用正交试验法分析比较乙醇体积分数、料液比例、水浴温度和提取时间对总黄酮提取量的影响。结果表明:各因素的影响大小依次为:提取温度>乙醇体积分数>提取时间>料液比例;马兰中总黄酮提取的最佳工艺为,乙醇体积分数75%,料液比例(g∶mL)1∶30,水浴温度70℃,提取时间90min,在该工艺下测得马兰总黄酮含量为16.452%;从3月～7月,马兰同一部位总黄酮含量存在显著差异性(P<0.05),7月份达最高值,叶为37.076%;茎次之,为8.501%;根最少,为4.314%。同时,同一月份中马兰不同部位总黄酮含量也存在显著差异性(P<0.05),其中叶中总黄酮含量最高。

Optimization of Extraction Technology of Total Flavonoid and Determination of Kalimeris indica in Different Sections

The total flavonoids of Kalimeris indica were extracted by the ultrasonic wave.At the same time,the orthogonal experiment was used to analyze the effects of alcohol concentration,material-solvent ratio,temperature and extraction time on the yield of total flavonoids.The order of the above four factors which affected extraction was temperature,alcohol concentration,extraction time and material-sovent ratio.The optimal conditions were 75%(volume fraction) alcohol solution,material-solvent ratio 1∶30(g/mL),extraction temperature 70℃ and extraction time 90 minutes.The yield of the crude flavonoids was 16.452%(mass fraction).The content of total flavonoid showed a large difference in the same section of Kalimeris indica from March to July.The highest was in July,37.076% was in leaves,8.501% in stem,and 4.314% in roots.Certain difference also existed among different parts of Kalimeris indica,flavonoids(P<0.05) was found the highest in leaves.