Antibiotics production of cellulosic waste with solid state fermentation by Streptomyces

Cellulosic waste, corncob, was used as a substrate in the production of oxytetracycline by Streptomyces rimosus TM-55 in solid state fermentation. Oxytetracycline was detected on the fourth day, and reached its maximum on the eighth day. During cultivation, the moisture content of substrate increased as incubation being, and pH value increased slightly. Optimal conditions for oxytetracycline production were an initial pH of 5.2 to 6.3, an initial moisture content of 64 to 67%, supplemented with 20% (w/w) rice bran or 1.5 to 2.5% (NH₄)₂SO₄ as the sole nitrogen source, 1.0% CaCO₃, 2% MgSO₄ 7H₂O, 0.5% KH₂PO₄, and 0.6 to 0.8% aspartic acid or lysine, with incubation for 8 days at 25 to 30°C. Each gram of substrate produced 10 to 11 mg of oxytetracycline.