河鲀Ⅰ型胶原蛋白提取物防治环磷酰胺、顺铂或阿糖胞苷诱发小鼠骨髓抑制的研究

目的:观察河Ⅰ 型胶原蛋白提取物(Pufferfish type Ⅰ collagen extract, PTICE) 对环磷酰胺(CTX) 、顺铂(DDP) 或阿糖胞苷(Ara-c) 诱发的小鼠骨髓抑制的影响。方法:分别用CTX 、DDP 、Arac腹腔注射(i.p.) 诱发小鼠骨髓抑制、免疫抑制等毒副反应, PTICE 灌胃(i.g.) 预防治疗, 观察小鼠的一般状况, 测定外周血细胞分类、脾脏指数和骨髓DNA 含量。结果:PTICE 可改善应用上述化疗药物小鼠的一般状况, 保护小鼠毛发, 升高外周血中的白细胞、血小板、中性粒细胞、淋巴细胞数,增加脾脏指数和骨髓DNA 含量;PTICE 显著降低DDP 导致的小鼠死亡率。结论:PTICE 可降低化疗药物引起的小鼠骨髓抑制等毒副作用。

Pufferfish type Ⅰ collagen extract prevented and cured myelosuppression induced by cyclophosphamide or diamminedichloroplatinum or cytosine arabinoside in mice

AIM:To observe the effects of Pufferfish type Ⅰ collagen extract (PTICE) on mouse toxic and adverse reactions induced by cyclophosphamide (CTX) or diamminedichloroplatinum (DDP) or cytosine arabinoside (Ara-c). METHODS:Mouse myelosuppression model was induced by i. p. CTX or DDP or Ara-c, i. g. PTICE to prevent and cure. The mental status, level of body weight and pelage were observed. The peripheral hemogram, spleen index, and contents of bone marrow DNA were determined. RESULTS:PTICE could obviously improve the mental status, protect the pelage, raise the counts of white blood cell, platelet, neutrophil, lymphocyte and increase the spleen index and contents of bone marrow DNA. PTICE decreased the mice mortality rate induced by DDP. CONCLUSION:PTICE could reduce myelosuppression induced by cyclophosphamide or diamminedichloroplatinum or cytosine arabinoside in mice.