Cytoprotective activity of lotus (Nelumbo nucifera Gaertner) leaf extracts on the mouse embryonic fibroblast cell |
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Authors: | Ju Yeon Ha Kyueun Lee Jeongmi Park Ahyoung Dong Han-Seung Shin |
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Affiliation: | (1) Department of Food and Nutrition, Inha University, Incheon, Korea;(2) Department of Chemistry, Konkuk University, Seoul, Korea |
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Abstract: | This study was conducted to evaluate the cytoprotective activity of lotus (Nelumbo nucifera Gaertner) leaf extract (LLE) on mouse embryonic fibroblast (MEF) cells. The 2-diphenyl-1-picrylhydrazyl hydrate (DPPH) free
radical scavenging activities of LLE increased in a concentration dependent manner. The cells, damaged by oxidative stress,
decreased their viability following increasing concentration of H2O2, but the co-treatment of n-butanol fraction of LLE and H2O2 resulted in an increase in cell growth, by about 25%, compared to the cells treated with H2O2. The n-butanol fraction of LLE inhibited the cytotoxicity induced by H2O2 in a concentration dependent manner. The oxidative damage to the cells, measured by apoptotic and necrotic cell accumulation,
was similar with the addition of the n-butanol fraction of LLE to H2O2. Taken together, these results suggest that LLE inhibited the cytotoxicity which is induced by H2O2, and has a protective effect on MEF cell against oxidative stress. |
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