Fractionation of non-polar compounds of basil (Ocimum basilicum L.) by supercritical fluid extraction (SFE) |
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| Affiliation: | 1. Technical Faculty “Mihajlo Pupin” Zrenjanin, University of Novi Sad, Djure Djakovica b.b., 23000 Zrenjanin, Serbia;2. Faculty of Technology, Department of Biotechnology and Pharmaceutical Engineering, University of Novi Sad, Boulevard Cara Lazara 1, 21000 Novi Sad, Serbia;3. Institute of Field and Vegetable Crops, Maksima Gorkog 30, 21000 Novi Sad, Serbia;1. University of Belgrade, Faculty of Technology and Metallurgy, Karnegijeva 4, 11120 Belgrade, Serbia;2. Institute for Medical Plant Research “Dr Josif Pancic”, Tadeusa Koscuska 1, 11000 Belgrade, Serbia;1. Department of Food Engineering, University of Campinas—UNICAMP, 13083-862 Campinas, SP, Brazil;2. Chemical, Biological and Agricultural Pluridisciplinary Research Center (CPQBA), State University of Campinas—UNICAMP, 13083-970 Campinas, SP, Brazil;3. ThoMSon Mass Spectrometry Laboratory, Institute of Chemistry, State University of Campinas—UNICAMP, 13083-862 Campinas, SP, Brazil;4. Laboratory of Virology, Institute of Biology, State University of Campinas—UNICAMP, 13084-971 Campinas, SP, Brazil;1. Department of Food Engineering, IFRS - Campus Erechim, Erechim, RS 99713–028, Brazil;2. Department of Food Technology, IFRS - Campus Sertão, Sertão, RS 99170–000, Brazil;3. Department of Engineering, UFFS, Chapecó, SC 99700-000, Brazil;4. Department of Biotechnology IFRS – Campus Porto Alegre, RS 90.030–041, Brazil;5. Center for Study on Colloidal Systems, ITP/UNIT, Aracaju, SE 49032–490, Brazil;1. Josip Juraj Strossmayer University of Osijek, Faculty of Food Technology Osijek, Franje Kuhača 20, 31000 Osijek, Croatia;2. Croatian Veterinary Institute, Branch – Veterinary Institute Vinkovci, Josipa Kozarca 24, 32100 Vinkovci, Croatia;3. Faculty of Chemistry and Technology, University of Split, R. Boškovića 35, 21000, Split, Croatia;1. Department of Food Engineering, State University of Campinas – UNICAMP, 13083-862 Campinas, SP, Brazil;2. Chemical, Biological and Agricultural Pluridisciplinary Research Center (CPQBA), State University of Campinas – UNICAMP, 13083-970 Campinas, SP, Brazil;3. ThoMSon Mass Spectrometry Laboratory, Institute of Chemistry, State University of Campinas – UNICAMP, 13083-970 Campinas, SP, Brazil |
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| Abstract: | Fractionation by supercritical fluid extraction (SFE) was used to obtain various extracts from Ocimum basilicum L. (sweet basil). The extractions were done at temperature of 40 °C and 50 °C, and at different pressure, which was increased successively from 100 bar (150 bar, 200 bar) to 300 bar. The identification and quantification of the extract compounds was done using gas chromatography–mass spectrometry (GC–MS) and gas chromatography with flame-ionization detector (GC–FID) analyses. The yield of basil SFE extracts varies from 0.138 to 1.008% (w/w). The major components identified in the extracts were linalool, eugenol, α-bergamotene, germacrene D, γ-cadinene, δ-cadinene, β-selinene and spathulenol. The highest extraction yield, in fractionation process, of linalool (167.03 mg per 100 g of basil) was achieved at pressure of 100 bar and temperature 50 °C (solvent density 0.378 kg/m3). |
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| Keywords: | SFE Extracts GC–MS analysis |
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