| 紫外线与N~+注入复合诱变选育曲酸高产菌株 |
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| 引用本文： | 凌帅,刘咏,姚建铭,李俊.紫外线与N~+注入复合诱变选育曲酸高产菌株[J].食品科学,2013,34(1):234-238. |
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| 作者姓名： | 凌帅 刘咏 姚建铭 李俊 |
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| 作者单位： | 合肥工业大学生物与食品工程学院;中国科学院合肥物质科学研究院 |
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| 摘 要： | 为得到一株曲酸的高产菌株,从5种实验菌株中筛选得到1株米曲霉CICC-2336菌株,以其作为出发菌株,经过紫外诱变和氮离子注入诱变两种复合诱变,得到1株曲酸高产菌株,将其命名为Aspergillus oryzaeN11。该菌株经过传代6次后,性状稳定,产酸量基本不变。在接种量15%、30℃恒温、摇床180r/min条件下发酵培养7d后,其产酸量达到24.12g/L,比出发菌株提高101%。
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| 关 键 词： | 米曲霉 曲酸 紫外诱变 氮离子注入诱变 传代 |
Mutation Breeding of High-Yield Kojic Acid-Producing Strains by UV Irradiation Combined with N~+ Implantation |
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| LING Shuai,LIU Yong,YAO Jian-ming,LI Jun.Mutation Breeding of High-Yield Kojic Acid-Producing Strains by UV Irradiation Combined with N~+ Implantation[J].Food Science,2013,34(1):234-238. |
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| Authors: | LING Shuai LIU Yong YAO Jian-ming LI Jun |
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| Affiliation: | 1.College of Food and Biological Engineering,Hefei University of Technology,Hefei 230009,China; 2.Hefei Institutes of Physical Science,Chinese Academy of Sciences,Hefei 230031,China) |
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| Abstract: | |
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| Keywords: | Aspergillus oryzae kojic acid UV-induced mutation N+ implantation-induced mutation subculture |
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