Synthesis of ethyl propionate catalyzed by poly(N‐AEAAm‐co‐AAc)‐cl‐MBAm hydrogel‐immobilized lipase of Bacillus coagulans MTCC‐6375 |
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| Authors: | Shamsher Singh Kanwar Rajeev Kumar Kaushal Amit Aggarwal Sandeep Chauhan Swapandeep Singh Chimni Ghansham Singh Chauhan |
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| Affiliation: | 1. Department of Biotechnology, Himachal Pradesh University, Summer Hill, Shimla‐171 005, India;2. Department of Biotechnology, University of Rajasthan, Jaipur, India;3. Department of Chemistry, Himachal Pradesh University, Summer Hill, Shimla‐171 005, India;4. Department of Chemistry, Guru Nanak Dev University, Amritsar‐143 005, India |
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| Abstract: | A purified alkaline thermotolerant bacterial lipase of Bacillus coagulans MTCC‐6375 was efficiently immobilized onto poly(N‐AEAAm‐co‐AAc‐cl‐MBAm)‐hydrogel at pH 8.5 and at temperature 55°C in 16 h. The hydrogel‐bound matrix possessed 1.04 U/g (matrix) lipase activity with a specific activity of 1.8 U/mg of protein. The immobilized lipase resulted in formation of 52.5 mM of ethyl propionate (52% conversion) at 55°C in 9 h in n‐nonane. Ethanol and propionic acid when used in a ratio of 300 : 100 mM, respectively, in n‐nonane along with 10 mg of hydrogel‐bound lipase resulted in optimal synthesis of ethyl propionate (82.5 mM). Addition of molecular sieves (3 Å, 0.7 g/reaction volume) further enhanced the conversion rate to 82.4% resulting in 83.5 mM of ethyl propionate. Incubation temperature below or above 55°C had a marked effect on the synthesis of ethyl propionate. However, esterification performed in n‐heptane at 65°C resulted in 87.5 mM of ethyl propionate with a conversation rate of 89.3%. © 2007 Wiley Periodicals, Inc. J Appl Polym Sci 2007 |
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| Keywords: | hydrogels lipase esterification |
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