| 枯草芽孢杆菌SH7 抑菌蛋白产生的最佳发酵条件及温室防效 |
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| 引用本文： | 张秀玉,孔凡玉,王静,张成省,李佰乐,杨斌,管志坤,王秀萍.枯草芽孢杆菌SH7 抑菌蛋白产生的最佳发酵条件及温室防效[J].中国烟草科学,2010,31(2):45-48. |
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| 作者姓名： | 张秀玉 孔凡玉 王静 张成省 李佰乐 杨斌 管志坤 王秀萍 |
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| 作者单位： | 1. 中国农业科学院烟草研究所，青岛 266101；2. 中国农业科学院研究生院，北京 100081；3. 山东青岛烟草有限公司，青岛 266071；4. 山东青岛烟草有限公司胶南分公司，山东 胶南 266400 |
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| 摘 要： | 枯草芽孢杆菌SH7可抑制烟草青枯病菌的生长，利用硫酸铵沉淀法从发酵液中提取抑菌蛋白质，测定其对烟草青
枯病菌抑菌活性，从而确定抑菌蛋白产生的最佳培养基和培养条件。本实验产生抗菌蛋白的最佳培养基为NA液体培养基，
最佳发酵条件为：pH 6.0，26 ℃，装液量100 mL，170 r/min振荡培养96 h。粗抑菌蛋白在pH为9.0和10.0时，其抑菌活性仍
保持为86%, 88%，对碱稳定；在100 ℃和121 ℃处理20 min，其抑菌活性仍保持为83.1%, 68.0%，具有良好的热稳定性。在
温室内，抗菌蛋白粗提液对烟草青枯病可取得70.8%的防效。
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| 关 键 词： | 枯草芽孢杆菌 最佳发酵条件 抑菌蛋白 活性 温室防效 |
| 收稿时间： | 2009-01-09 |
Optimal Fermentative Condition of Antagonistic Protein Produced by Antagonistic SH7 and its Control Effects in Greenhouse |
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| ZHANG Xiuyu,KONG Fanyu,WANG Jing,ZHANG Chengsheng,LI Baile,YANG Bin,GUAN Zhikun,WANG Xiuping.Optimal Fermentative Condition of Antagonistic Protein Produced by Antagonistic SH7 and its Control Effects in Greenhouse[J].Chinese Tobacco Science,2010,31(2):45-48. |
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| Authors: | ZHANG Xiuyu KONG Fanyu WANG Jing ZHANG Chengsheng LI Baile YANG Bin GUAN Zhikun WANG Xiuping |
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| Affiliation: | 1. Tobacco Research Institute of CAAS, Qingdao 266101, China; 2. Graduate School of CAAS, Beijing 100081, China; 3. Qingdao;
Tobacco Co. Ltd., Qingdao 266071, China; 4. Jiaonan Branch, Qingdao Tobacco Co. Ltd., Jiaonan Shandong 2664000, China |
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| Abstract: | Bacillus subtilis SH7 can inhibit the growth of tobacco Ralstonia solanacearum. In order to investigate the best medium
and optimal fermentative condition, antagonistic protein was extracted from fermentative optimal medium by using ammonium
sulphate precipitation method, and its antagonistic ability to tobacco Ralstonia solanacearum was measured. Results showed that
optimal medium for SH7 was NA medium, and its optimal fermentative condition was pH 6.0, 26 ℃, 100 mL/250mL, 170 r/min, 96
h shaking culture. The antagonistic protein was insensitive to alkaline, and at pH 9.0 or 10.0, its antagonistic ability was 86% and
88%, respectively. At 100 and 121 ℃, 20 min, antagonistic ability was 83.1% and 68.0%, respectively. The control effect of the crude
protein to tobacco bacterial wilt in greenhouse was 70.8% by injection． |
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| Keywords: | Bacillus subtilis optimal fermentative condition antagonistic protein activity control effect in greenhouse |
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