| 猪瘟疫苗免疫反应低下猪CD3~+CD4~+和CD4~+CD25~+淋巴细胞亚群分析 |
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| 引用本文： | 王惟,杨知,付明山,郑海英,李芳萍,涂长春.猪瘟疫苗免疫反应低下猪CD3~+CD4~+和CD4~+CD25~+淋巴细胞亚群分析[J].粉末涂料与涂装,2010,23(4). |
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| 作者姓名： | 王惟 杨知 付明山 郑海英 李芳萍 涂长春 |
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| 作者单位： | 王惟,杨知,付明山(吉林大学畜牧兽医学院,长春,130062;军事医学科学院军事兽医研究所,长春,130062);郑海英,李芳萍(通辽市畜牧兽医科研所,内蒙古通辽,028042);涂长春(军事医学科学院军事兽医研究所,长春,130062) |
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| 摘 要： | 目的分析猪瘟疫苗免疫反应低下猪CD3+CD4+和CD4+CD25+淋巴细胞亚群的分布。方法将接种过猪瘟疫苗的种母猪经ELISA和血清中和试验检测筛选出猪瘟病毒(CSFV)抗体阴性猪。从中挑选出15头作为研究对象,以15头抗体阳性猪作为对照。采集猪的抗凝血,分离外周血淋巴细胞(PBL),流式细胞术检测30头猪PBL中CD3+CD4+和CD4+CD25+淋巴细胞亚群的分布。结果CSFV抗体阴性猪PBL中CD3+CD4+细胞的比例(24.09%±1.29%)明显低于CSFV抗体阳性猪(37.49%±1.60%);CSFV抗体阴性猪PBL中CD4+CD25+细胞的比例(2.34%±0.20%)明显高于CSFV抗体阳性猪(1.64%±0.13%)。结论PBL中CD3+CD4+淋巴细胞亚群的减少及CD4+CD25+淋巴细胞亚群的增加,可能是导致猪瘟疫苗免疫反应低下的重要原因。
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| 关 键 词： | 猪瘟病毒 外周血淋巴细胞 CD3+CD4+亚群 CD4+CD25+亚群 |
Analysis of CD3~+CD4~+ and CD4~+CD25~+ Lymphocyte Subsets of Pigs with Dumbing Immune Response to Classical Swine Fever Vaccine |
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| WANG Wei,YANG Zhi,FU Ming-shan,et al.Analysis of CD3~+CD4~+ and CD4~+CD25~+ Lymphocyte Subsets of Pigs with Dumbing Immune Response to Classical Swine Fever Vaccine[J].Chinese Journal of Biologicals,2010,23(4). |
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| Authors: | WANG Wei YANG Zhi FU Ming-shan |
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| Affiliation: | WANG Wei,YANG Zhi,FU Ming-shan,et al (College of Animal Science , Veterinary Medicine,Jilin University,Changchun 130062,China) |
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| Abstract: | Objective To analyze the distribution of CD3+CD4+ and CD4+CD25+ lymphocyte subsets in the pigs with dumbing immune response to classical swine fever(CSF)vaccine. Methods Fifteen CSF virus(CSFV)antibody-negative pigs were screened by ELISA and serum neutralizing antibody test from the female breeding pigs immunized with CSF vaccine, using 15 antibody-positive pigs as control. The anticoagulant blood of pigs was collected for isolation of peripheral blood lymphocytes(PBLs)in which the distribution of CD3+CD4+... |
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| Keywords: | Classical swine fever virus(CSFV) Peripheral blood lymphocytes(PBLs) CD3~+CD4~+ subset CD4~+CD25~+ subset |
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